Effects of superoxide anion on intracellular Ca2+ concentration in rabbit pulmonary arterial smooth muscle cells
文献类型：期刊论文
| 作者 | Wang, YP ; Oike, M; Ito, Y
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999-01 |
| 卷号 | 20期号:1页码:10-14 |
| 关键词 | calcium cell separation vascular smooth muscle pulmonary artery free radicals xanthines xanthine oxidase adenosine triphosphate thapsigargin |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the effect of superoxide anion on the Ca2+ homeostasis in smooth muscle cells isolated from the rabbit pulmonary artery. METHODS: Intracellular Ca2+ concentration ([Ca2+](i)) was investigated using cell suspension of freshly isolated smooth muscle cells from rabbit pulmonary artery (PASMC). Fura-2 fluorescent ratio obtained at 340 nm and 380 nm wave lengths was measured as an indicator of [Ca2+](i). RESULTS: ATP 30 mu mol.L-1 induced a transient increase in the ratio (Ca2+ transient). Thapsigargin, an inhibitor of sarcoplasmic Ca2+ ATPase, induced a phasic increase in the ratio due to Ca2+ leak from intracellular store sites, but not the sustained increase, thereby suggesting the absence of Ca2+ release-activated Ca2+ entry (CRAC) mechanism in PASMC. When PASMC were exposed to superoxide anion by the pretreatment with xanthine and xanthine oxidase (X/XO) for 30 min, sustained component of ATP-induced Ca2+ transient was elevated. The ratios at 5 and 10 min after ATP application (Delta ratio(5) (min) and Delta ratio(10) (min)) were increased from 0.091 +/- 0.022 to 0.149 +/- 0.048 (P < 0.05) and from 0.021 +/- 0.020 to 0.117 +/- 0.047 (P < 0.01), respectively. But, thapsigargin-induced [Ca2+](i) transient was not affected by X/XO. CONCLUSION: Superoxide anion makes ATP-induced Ca2+ transient sluggish, and does not affect Ca2+ leak pathway in PASMC. |
| WOS关键词 | OSCILLATIONS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000077978900002 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274768]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, YP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China 2.Kyushu Univ, Fac Med, Dept Pharmacol, Fukuoka 81282, Japan |
| 推荐引用方式 GB/T 7714 | Wang, YP,Oike, M,Ito, Y. Effects of superoxide anion on intracellular Ca2+ concentration in rabbit pulmonary arterial smooth muscle cells[J]. ACTA PHARMACOLOGICA SINICA,1999,20(1):10-14. |
| APA | Wang, YP,Oike, M,&Ito, Y.(1999).Effects of superoxide anion on intracellular Ca2+ concentration in rabbit pulmonary arterial smooth muscle cells.ACTA PHARMACOLOGICA SINICA,20(1),10-14. |
| MLA | Wang, YP,et al."Effects of superoxide anion on intracellular Ca2+ concentration in rabbit pulmonary arterial smooth muscle cells".ACTA PHARMACOLOGICA SINICA 20.1(1999):10-14. |
入库方式： OAI收割
来源：上海药物研究所
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