Preparation of monoclonal antibody against crocin and its characterization
文献类型：期刊论文
| 作者 | Xuan, LJ ; Tanaka, H; Xu, YM; Shoyama, Y
|
| 刊名 | CYTOTECHNOLOGY
 |
| 出版日期 | 1999 |
| 卷号 | 29期号:1页码:65-70 |
| 关键词 | crocetin glycosides crocin Crocus sativus ELISA MALDI-TOF-MS monoclonal antibody |
| ISSN号 | 0920-9069 |
| DOI | 10.1023/A:1007993615489 |
| 文献子类 | Article |
| 英文摘要 | Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b) possessing lambda light chain, respectively. Their wide reactivities against crocetin glycosides were discussed. |
| WOS关键词 | IONIZATION MASS-SPECTROMETRY ; LONG-TERM POTENTIATION ; ETHANOL-INDUCED IMPAIRMENT ; SERUM-ALBUMIN CONJUGATE ; SATIVUS L ; IN-VIVO ; CELLS ; CROCETIN ; SAFFRON ; EXTRACT |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000080237200007 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274770]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xuan, LJ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200032, Peoples R China 2.Kyushu Univ, Fac Pharmaceut Sci, Dept Pharmacognosy, Higashi Ku, Fukuoka 812, Japan |
| 推荐引用方式 GB/T 7714 | Xuan, LJ,Tanaka, H,Xu, YM,et al. Preparation of monoclonal antibody against crocin and its characterization[J]. CYTOTECHNOLOGY,1999,29(1):65-70. |
| APA | Xuan, LJ,Tanaka, H,Xu, YM,&Shoyama, Y.(1999).Preparation of monoclonal antibody against crocin and its characterization.CYTOTECHNOLOGY,29(1),65-70. |
| MLA | Xuan, LJ,et al."Preparation of monoclonal antibody against crocin and its characterization".CYTOTECHNOLOGY 29.1(1999):65-70. |
入库方式： OAI收割
来源：上海药物研究所
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