The C-truncated delta-opioid receptor underwent agonist-dependent activation and desensitization
文献类型：期刊论文
| 作者 | Wang, CH ; Zhou, DH; Cheng, ZJ; Wei, Q; Chen, J; Li, GF; Pei, G; Chi, ZQ
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 1998-08-19 |
| 卷号 | 249期号:2页码:321-324 |
| ISSN号 | 0006-291X |
| DOI | 10.1006/bbrc.1998.9080 |
| 文献子类 | Article |
| 英文摘要 | The agonist-dependent activation and desensitization of a recombinant analog of delta-opioid receptor lacking the carboxyl-terminal (C-terminal) 31 residue peptide segment were discussed. The cDNA of the C-truncated delta-opioid receptor was created by polymerase chain reaction (PCR), stably expressed in Chinese hamster ovary cells and characterized by binding assay and function assay. Agonist [D-Pen(2), D-Pen(5)]-enkephalin (DPDPE) stimulated the specific [S-35]GTP gamma S binding to membrane fragments of cells expressing the C-truncated and the wild-type delta-opioid receptors in different levels dose-dependently. EC50 values of DPDPE on truncated and wild-types in stimulation were very similar. Agonist-dependent desensitization, which could be blocked by protein kinase inhibitor staurosporine (Stau), was observed after pretreating truncated receptors with 1 mu M DPDPE for 10 min, the same as wild-types. The results reveal that the C-terminal of the delta-opioid receptor is not involved in controlling the G-protein activation and phosphorylation-related functional desensitization. (C) 1998 Academic Press. |
| WOS关键词 | COOH-TERMINAL TAIL ; MORPHINE-TOLERANCE ; OPIATE-RECEPTOR ; PHOSPHORYLATION ; SEQUESTRATION ; TRAFFICKING ; MECHANISMS ; SIGNAL ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000075515900005 |
| 出版者 | ACADEMIC PRESS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274813]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhou, DH |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, CH,Zhou, DH,Cheng, ZJ,et al. The C-truncated delta-opioid receptor underwent agonist-dependent activation and desensitization[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,1998,249(2):321-324. |
| APA | Wang, CH.,Zhou, DH.,Cheng, ZJ.,Wei, Q.,Chen, J.,...&Chi, ZQ.(1998).The C-truncated delta-opioid receptor underwent agonist-dependent activation and desensitization.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,249(2),321-324. |
| MLA | Wang, CH,et al."The C-truncated delta-opioid receptor underwent agonist-dependent activation and desensitization".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 249.2(1998):321-324. |
入库方式： OAI收割
来源：上海药物研究所
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