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Binding properties of C-truncated delta opioid receptors

文献类型：期刊论文

作者Wang, CH; Zhou, DH; Chen, J; Li, GF; Pei, G; Chi, ZQ
刊名ACTA PHARMACOLOGICA SINICA
出版日期1997-07
卷号18期号:4页码:337-340
关键词delta opioid receptors mutation CHO cells diprenorphine, leucine-2-alanine enkephalin, binding sites radioligand assay
ISSN号0253-9756
文献子类Article
英文摘要AIM: To study the role of C-terminal delta opioid receptor involved in ligand binding affinity and selectivity. METHODS: The 31 amino acid residues of C-terminal truncated delta opioid receptors and the wild-type were expressed stably in Chinese hamster ovary (CHO) cells, respectively. Then the ligand binding properties of the products were studied by receptor binding assay. RESULTS: A typical mutated receptor clone CHO-T and a wild-type receptor clone CHO-W were obtained. The K-d values of [H-3] diprenorphine (Dip) and [H-3]leucine-2-alanine enkephalin (DADLE) bound to CHO-T were similar to CHO-W. Both the specific [H-3]Dip bindings of CHO-T and CHO-W were strongly inhibited by delta selective agonists with similar K-i, but neither by mu nor kappa selective agonists. CONCLUSION: The C-terminal of the delta opioid receptor is not involved in the ligands binding affinity and selectivity.
WOS关键词INTERNALIZATION ; EXPRESSION
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:411864
WOS记录号WOS:A1997XJ69800012
出版者ACTA PHARMACOLOGICA SINICA
源URL[http://119.78.100.183/handle/2S10ELR8/274899]  
专题中国科学院上海药物研究所
作者单位1.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA
2.CHINESE ACAD SCI,SHANGHAI INST CELL BIOL,SHANGHAI 200031,PEOPLES R CHINA
推荐引用方式
GB/T 7714		 Wang, CH,Zhou, DH,Chen, J,et al. Binding properties of C-truncated delta opioid receptors[J]. ACTA PHARMACOLOGICA SINICA,1997,18(4):337-340.
APA Wang, CH,Zhou, DH,Chen, J,Li, GF,Pei, G,&Chi, ZQ.(1997).Binding properties of C-truncated delta opioid receptors.ACTA PHARMACOLOGICA SINICA,18(4),337-340.
MLA Wang, CH,et al."Binding properties of C-truncated delta opioid receptors".ACTA PHARMACOLOGICA SINICA 18.4(1997):337-340.

入库方式： OAI收割

来源：上海药物研究所

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