Effect of DL111-IT on progesterone biosynthesis and viability of rat luteal cells in vitro
文献类型：期刊论文
| 作者 | Yang, B; Cao, L; Fang, RY; Gu, ZP |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1997-07 |
| 卷号 | 18期号:4页码:367-370 |
| 关键词 | DL111-IT corpus luteum progesterone pregnenolone forskolin cultured cells cell survival chorionic gonadotropins |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the influence of DL111-IT on progesterone biosynthesis of cultured luteal cells (LC). METHODS: LC viability was assessed with trypan blue dye exclusion and progesterone concentration was measured with radioimmunoassay. RESULTS: DL111-IT decreased the viability of LC after 24-h incubation, its ED50 being 7.7 (95 % confidence limits: 7.1 - 8.5) mg.L-1. DL111-IT inhibited basal secretion of progesterone in a concentration-dependent manner, and 3 mg.L-1 decreased progesterone concentration by 25 % vs control. DL111-IT 3 mg.L-1 also inhibited the stimulatory effect of forskolin (cAMP activator) 10 mu mol.L-1 and pregnenolone [converted to progesterone by 3 beta-hydroxysteroid dehydrogenase-isomerase complex (3 beta-HSD)] 10 mu mol.L-1 on progesterone production in cultured LC, and their inhibitory rates were 43 % and 155 %, respectively. At the same concentration, DL111-IT did not influence hCG-induced progesterone production. CONCLUSION: DL111-IT inhibited progesterone synthesis by suppressing the conversion of pregnenolone to progesterone (inactivating 3 beta-HSD) and suppressed the activity of cAMP. DL111-IT 6 - 24 mg.L-1 decreased the viability of LC. |
| WOS关键词 | GOSSYPOL |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997XJ69800020 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274900]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, B |
| 作者单位 | ZHEJIANG MED UNIV,SCH PHARM,DEPT PHARMACOL,HANGZHOU 310031,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Yang, B,Cao, L,Fang, RY,et al. Effect of DL111-IT on progesterone biosynthesis and viability of rat luteal cells in vitro[J]. ACTA PHARMACOLOGICA SINICA,1997,18(4):367-370. |
| APA | Yang, B,Cao, L,Fang, RY,&Gu, ZP.(1997).Effect of DL111-IT on progesterone biosynthesis and viability of rat luteal cells in vitro.ACTA PHARMACOLOGICA SINICA,18(4),367-370. |
| MLA | Yang, B,et al."Effect of DL111-IT on progesterone biosynthesis and viability of rat luteal cells in vitro".ACTA PHARMACOLOGICA SINICA 18.4(1997):367-370. |
入库方式： OAI收割
来源：上海药物研究所
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