Dual actions of (-)-stepholidine on the dopamine receptor-mediated adenylate cyclase activity in rat corpus striatum
文献类型：期刊论文
| 作者 | Dong, ZJ; Guo, X; Chen, LJ; Han, YF; Jin, GZ
|
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 1997-06-20 |
| 卷号 | 61期号:4页码:465-472 |
| 关键词 | (-)-stepholidine dopamine receptors adenylate cyclase corpus striatum |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/S0024-3205(97)00404-9 |
| 文献子类 | Article |
| 英文摘要 | (-)-Stepholidine (SPD) is an antagonist of normosensitive dopamine (DA) receptors, but it exhibits D-1 agonistic action on rotational behaviour in rats with unilateral 6-hydroxydopamine (6-OHDA) lesions of the substantia nigra pars compacta (SNC). In the present study, agonistic and antagonistic effects of SPD on the DA receptor-mediated synaptosomal adenylate cyclase (AC) activity in rat striatum were investigated. After blockade of D-2 receptors, SPD augmented AC activity dose-dependently. The EC50 value was 41.1 +/- 8.6 mu mol/L. At the concentration of 10 mu mol/L, SPD increased cAMP formation from a basal level (50.8 +/- 10.3 pmol/mg protein/min) to 133.7 +/- 31.8 pmol/mg protein/min. The SPD-induced stimulation of AC activity was almost completely reversed by 10 mu mol/L Sch23390. These results indicate that SPD possesses an agonistic action on the D-1 receptor. Forskolin-stimulated adenylate cyclase (FSAC) activity was used as a model to elucidate the effect of SPD on D-2 receptors. The results indicate that DA inhibited FSAC activity dose-dependently, while SPD partially restored FSAC activity. Taken together, these results support the conclusion that SPD has dual actions on DA receptors that mediate AC activity, i.e., an agonistic action on D-1 receptors and an antagonistic action on D-2 receptors. |
| WOS关键词 | TYROSINE-HYDROXYLASE ACTIVITY ; L-STEPHOLIDINE ; SUBTYPES ; ANALOGS ; BRAIN |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997XG97100014 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274903]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA 2.HONG KONG UNIV SCI & TECHNOL,DEPT BIOCHEM,HONG KONG,HONG KONG |
| 推荐引用方式 GB/T 7714 | Dong, ZJ,Guo, X,Chen, LJ,et al. Dual actions of (-)-stepholidine on the dopamine receptor-mediated adenylate cyclase activity in rat corpus striatum[J]. LIFE SCIENCES,1997,61(4):465-472. |
| APA | Dong, ZJ,Guo, X,Chen, LJ,Han, YF,&Jin, GZ.(1997).Dual actions of (-)-stepholidine on the dopamine receptor-mediated adenylate cyclase activity in rat corpus striatum.LIFE SCIENCES,61(4),465-472. |
| MLA | Dong, ZJ,et al."Dual actions of (-)-stepholidine on the dopamine receptor-mediated adenylate cyclase activity in rat corpus striatum".LIFE SCIENCES 61.4(1997):465-472. |
入库方式： OAI收割
来源：上海药物研究所
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