Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons
文献类型：期刊论文
| 作者 | Wu, J; Jin, GZ
|
| 刊名 | NEUROSCIENCE LETTERS
 |
| 出版日期 | 1997-01-31 |
| 卷号 | 222期号:2页码:115-118 |
| 关键词 | tetrahydroberberine acetylcholine K+ current rat hippocampus dissociated neurons patch-clamp |
| ISSN号 | 0304-3940 |
| DOI | 10.1016/S0304-3940(97)13356-0 |
| 文献子类 | Article |
| 英文摘要 | The effects of a novel chemical type of dopamine receptor antagonist, the tetrahydroprotoberberine analogs (THPBs), on acetylcholine (ACh)-induced current were studied in freshly dissociated pyramidal neurons from rat hippocampal CA1 area using the nystatin perforated patch-clamp recording technique. Under voltage clamp conditions, the ACh-induced outward current (I-ACh) is sensitive to the muscarinic receptor antagonist, atropine and the K+ channel blocker, TEA. The reversal potential of I-ACh(-84.1+/-0.8 mV) is close to the K+ equilibrium potential, indicating that the I-ACh is mediated by a muscarinic receptor, and is carried mainly by K+. Tetrahydroberberine (THB) markedly reduced the I-ACh while its chemical analogs, l-stepholidine (l-SPD) or l-tetrahydropalmatine (l-THP), had little effect on the I-ACh. The half-maximal inhibitory concentration (IC50) of THB was 1.3x10(-5) M for a 10(-5) M ACh-induced I-ACh. THB suppressed the maximum of the ACh concentration-response curve without shifting the Hill coefficient, indicating a non-competitive inhibition. It is concluded that THE non-competitively inhibits the ACh-induced K+ current in a concentration-dependent manner, and that this inhibitory effect provides further evidence that THE plays its pharmacological roles in the central nervous system by effects other than through blockade of dopamine receptors. (C) 1997 Elsevier Science Ireland Ltd. |
| WOS关键词 | L-TETRAHYDROPALMATINE ; L-STEPHOLIDINE ; DOPAMINE ; TETRAHYDROPROTOBERBERINES ; (-)-STEPHOLIDINE ; STRIATUM ; ARTERY ; CELLS |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997WH24700012 |
| 出版者 | ELSEVIER SCI IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274932]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.UNIV NEW MEXICO,SCH MED,DEPT NEUROL,ALBUQUERQUE,NM 87131 2.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 20031,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Wu, J,Jin, GZ. Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons[J]. NEUROSCIENCE LETTERS,1997,222(2):115-118. |
| APA | Wu, J,&Jin, GZ.(1997).Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons.NEUROSCIENCE LETTERS,222(2),115-118. |
| MLA | Wu, J,et al."Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons".NEUROSCIENCE LETTERS 222.2(1997):115-118. |
入库方式： OAI收割
来源：上海药物研究所
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