Induction of apoptosis in human leukemia K562 cells by alpha-anordrin
文献类型：期刊论文
| 作者 | Lou, LG ; Xu, B
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1996-05 |
| 卷号 | 17期号:3页码:255-& |
| 关键词 | alpha-anordrin apoptosis leukemia K562 postcoital contraceptives cultured tumor cells phase-contrast microscopy DNA damage |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study antitumor action of alpha-anordrin (Ano). METHODS: Morphological assessment of apoptosis was performed with light microscope and electron microscope. Membrane integrity was determined by trypan blue exclusion method. Endonucleolysis was assessed by agarose gel electrophoresis and flow RESULTS: Exposure of K562 cells to Ano 2.5 - 50 mu mol . L(-1) for 48 h resulted in growth arrest, Ano 50 mu mol . L(-1) inhibited the growth of K562 cells by 67%. Cells were mainly blocked to progress through S-phase and arrested at G(1) phase. After treatment of K562 cells with Ano, marked morphological changes including condensed chromatin, nuclear fragmentation, and reduction in volume were observed. Agarose gel electrophoresis of DNA from cells treated with Ano for 24 - 48 h revealed ''ladder'' pattern, typical features of apoptosis, and near 70% of cells underwent apoptosis as determined by flow cytometry. The S-phase cells were more susceptible to apoptosis. Despite extensive cleavage of DNA and nuclear fragmentation, the cell membrane of Ano-treated cells remained intact, excluding trypan blue. Apoptotic cells were detected as early as 8 h after Ano (50 mu mol . L(-1)) treatment. CONCLUSION: Ano induces apoptosis in K562 cells. |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:339745 |
| WOS记录号 | WOS:A1996UK28100020 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274983]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Lou, LG |
| 作者单位 | CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA. |
| 推荐引用方式 GB/T 7714 | Lou, LG,Xu, B. Induction of apoptosis in human leukemia K562 cells by alpha-anordrin[J]. ACTA PHARMACOLOGICA SINICA,1996,17(3):255-&. |
| APA | Lou, LG,&Xu, B.(1996).Induction of apoptosis in human leukemia K562 cells by alpha-anordrin.ACTA PHARMACOLOGICA SINICA,17(3),255-&. |
| MLA | Lou, LG,et al."Induction of apoptosis in human leukemia K562 cells by alpha-anordrin".ACTA PHARMACOLOGICA SINICA 17.3(1996):255-&. |
入库方式： OAI收割
来源：上海药物研究所
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