Lead cytotoxicity in primary cultured rat astrocytes and schwann cells
文献类型：期刊论文
| 作者 | Tang, HW; Yan, HL; Hu, XH; Liang, YX; Shen, XY |
| 刊名 | JOURNAL OF APPLIED TOXICOLOGY
 |
| 出版日期 | 1996-05 |
| 卷号 | 16期号:3页码:187-196 |
| 关键词 | lead cytotoxicity astrocytes Schwann cells sulphydryl group lipid peroxidation lactic dehydrogenase dithiothreitol glutathione adenosine 3',5'-cyclic monophosphate |
| ISSN号 | 0260-437X |
| DOI | 10.1002/(SICI)1099-1263(199605)16:3<187::AID-JAT329>3.0.CO;2-Y |
| 文献子类 | Article |
| 英文摘要 | The cytotoxic effects of lead acetate on primary cultured astrocytes and Schwann cells (SCs) were studied for comparing the sensitivity of the two kinds of cells and exploring the possible mechanism of lead cytotoxicity. The results indicated that the number of astrocytes detached from the culture surfaces were dependent on the concentration and time of lead exposure, Under phase contrast microscopy, increases in the number of vacuoles were observed at doses of 50 and 100 mu g ml(-1) lead after 96 h of lead exposure for astrocytes and 1, 5 and 10 mu g ml(-1) lead after 24 h of lead exposure for SCs, By scanning electron microscopy, the surface changes in astrocytes began to appear at the dose of 10 mu g ml(-1) lead, whereas in SCs it began in 1 mu g ml(-1) lead, By transmission electron microscopy, astrocytes exposed to 10, 50 and 100 mu g ml(-1) lead had increased lysosomal densities, the presence of nuclear inclusions and enlargement of rough endoplasmic reticulum, whereas SCs exposed to 1 mu g ml(-1) lead began to show swelling of mitochondria and endoplasmic reticulum, cytoplasmic vacuolizations and numerous myelinoid bodies, Increases in the content of lactic dehydrogenase (LDH) leakage from the astrocytes exposed to 100 mu g ml(-1) lead and SCs exposed to 1, 5 and 10 mu g ml(-1) lead were observed, respectively, Decreases in sulphydryl group (SH) levels in astrocytes exposed to 50 and 100 mu g ml(-1) lead and SCs exposed to 1, 5 and 10 mu g ml(-1) lead were also observed, A dose of 1.0 mmol l(-1) reduced glutathione (GSH) and 2.0 mmol l(-1) dithiothreitol (DTT) could protect astrocytes from lead-induced SH decrease and LDH leakage, Doses of 10-100 mu mol l(-1) cAMP were shown to have a protective effect on lead-induced SH decrease in SCs, No significant changes of GSH or lipid peroxidation (LPO) were observed in astrocytes, The results indicated that SH was involved in lead-induced cytotoxicity of astrocytes and SCs, and SCs were more sensitive to lead-induced cytotoxicity than astrocytes. |
| WOS关键词 | CEREBRAL ASTROCYTES ; LIPID-PEROXIDATION ; GLUTATHIONE STATUS ; TOXICITY ; HEPATOCYTES ; INHIBITION ; ASTROGLIA ; MEMBRANES ; EXPOSURE ; CADMIUM |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:A1996UW50300001 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274984]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Tang, HW |
| 作者单位 | 1.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA 2.SHANGHAI MED UNIV,DEPT ANAT,SHANGHAI 200032,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Tang, HW,Yan, HL,Hu, XH,et al. Lead cytotoxicity in primary cultured rat astrocytes and schwann cells[J]. JOURNAL OF APPLIED TOXICOLOGY,1996,16(3):187-196. |
| APA | Tang, HW,Yan, HL,Hu, XH,Liang, YX,&Shen, XY.(1996).Lead cytotoxicity in primary cultured rat astrocytes and schwann cells.JOURNAL OF APPLIED TOXICOLOGY,16(3),187-196. |
| MLA | Tang, HW,et al."Lead cytotoxicity in primary cultured rat astrocytes and schwann cells".JOURNAL OF APPLIED TOXICOLOGY 16.3(1996):187-196. |
入库方式： OAI收割
来源：上海药物研究所
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