Tetrahydroberberine suppresses dopamine-induced potassium current in acutely dissociated CA1 pyramidal neurons from rat hippocampus
文献类型：期刊论文
| 作者 | Wu, J; Jin, GZ
|
| 刊名 | NEUROSCIENCE LETTERS
 |
| 出版日期 | 1996-04-05 |
| 卷号 | 207期号:3页码:155-158 |
| 关键词 | tetrahydroberberine dopamine K+ current rat hippocampus dissociated neurons patch-clamp |
| ISSN号 | 0304-3940 |
| DOI | 10.1016/0304-3940(96)12522-2 |
| 文献子类 | Article |
| 英文摘要 | Effects of tetrahydroberberine (THE) on dopamine (DA)-induced response have been investigated in single pyramidal neurons freshly dissociated from CA1 area of the hippocampus using the nystatin perforated patch-clamp, whole-cell recording technique under voltage-clamp configuration. At a holding potential (V-H) of -20 mV, DA-induced responses included a transient outward current, a slow inward current and a combination of the two. The outward current had a reversal potential of -83.5 +/- 8.0 mV which was close to K+ equilibrium potential and was sensitive to TEA, suggesting that this outward current was carried by K+. Application of THB reversibly suppressed three type responses induced by DA with different degrees of inhibitory ratio. THE inhibited the DA-induced outward current in a concentration-dependent manner with an IC50 of 13 mu M. The maximal value of the concentration-response curve for DA-induced outward current was suppressed by THE, suggesting a non-competitive inhibition. The results support the hypothesis that THE acts as a novel dopaminergic system antagonist. Furthermore, THE inhibits the DA-induced K+ current non-competitively in single dissociated cells, implying that THE may exhibit other pharmacological action on the central nervous system. |
| WOS关键词 | D-CAMPHOR SULFONATE ; TETRAHYDROPROTOBERBERINES ; RECEPTORS ; CELLS ; BRAIN |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:A1996UF98700004 |
| 出版者 | ELSEVIER SCI IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274987]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.UNIV NEW MEXICO,SCH MED,DEPT NEUROL,ALBUQUERQUE,NM 87131 2.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Wu, J,Jin, GZ. Tetrahydroberberine suppresses dopamine-induced potassium current in acutely dissociated CA1 pyramidal neurons from rat hippocampus[J]. NEUROSCIENCE LETTERS,1996,207(3):155-158. |
| APA | Wu, J,&Jin, GZ.(1996).Tetrahydroberberine suppresses dopamine-induced potassium current in acutely dissociated CA1 pyramidal neurons from rat hippocampus.NEUROSCIENCE LETTERS,207(3),155-158. |
| MLA | Wu, J,et al."Tetrahydroberberine suppresses dopamine-induced potassium current in acutely dissociated CA1 pyramidal neurons from rat hippocampus".NEUROSCIENCE LETTERS 207.3(1996):155-158. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。