Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS
文献类型：期刊论文
| 作者 | Wu, J; Chen, PX; Jin, GZ
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1996-01 |
| 卷号 | 17期号:1页码:23-& |
| 关键词 | dopamine potassium channels hippocampus pyramidal cells patch-clamp techniques |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To determine whether or not the dopamine (DA) can induce ionic current in single neuron acutely dissociated from different central areas including striatum, ventral tegmental area (VTA), substantia nigra pars compacta (SNC) and hippocampal CA(1) area. METHODS: Using a new patch-clamp whole-cell recording technic, namely nystatin-perforated whole-cell configuration under voltage-clamp mode. RESULTS: In 36 single neurons isolated-from the striatum and VTA, DA 10-1000 mu mol . L(-1) was quite diverse to elicit ionic current response. In 19 SNC neurons, 5 neurons (26%) response to 1 mmol . L(-1) DA represented as a small outward current (11.3 +/- 2.4 pA) at a holding potential (V-H) of - 20 mV. In. 25 of 69 (36%) examined hippocampal CA(1) pyramidal neurons, however, application of DA induced 3 types of current responses: outward current (8 neurons) accompanied with an increase of membrane conductance, slow inward current (5 neurons) with an decrease of membrane conductance and outward-following inward current (12 neurons) at a V-H of - 20 mV. The concentration-response relationship of DA-induced currents showed the typical sigmoid shape with the threshold dose, being the maxium response dose are 3 mu mol . L(-1) and 1 mmol . L(-1) respectively. The current-voltage (I-V) relationship of DA-induced responses did not show any voltage-dependent manner and the reversal potential (EDA) was close to the equilibrium potential of potassium (E(K)) calculated with the Nernst equation. TEA 5 mol . L(-1) effectively inhibited the DA-induced response. CONCLUSION: These results suggest that DA-induced outward current is carried by K+ in single hippocampel CA(1) pyramidal neuron. |
| WOS关键词 | HIPPOCAMPAL-FORMATION ; CORPUS STRIATUM ; RECEPTORS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:339684 |
| WOS记录号 | WOS:A1996TQ30000005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275009]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA 2.SUN YAT SEN UNIV MED SCI,DEPT PHYSIOL,CANTON 510089,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Wu, J,Chen, PX,Jin, GZ. Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS[J]. ACTA PHARMACOLOGICA SINICA,1996,17(1):23-&. |
| APA | Wu, J,Chen, PX,&Jin, GZ.(1996).Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS.ACTA PHARMACOLOGICA SINICA,17(1),23-&. |
| MLA | Wu, J,et al."Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS".ACTA PHARMACOLOGICA SINICA 17.1(1996):23-&. |
入库方式： OAI收割
来源：上海药物研究所
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