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Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS

文献类型:期刊论文

作者Wu, J; Chen, PX; Jin, GZ
刊名ACTA PHARMACOLOGICA SINICA
出版日期1996-01
卷号17期号:1页码:23-&
关键词dopamine potassium channels hippocampus pyramidal cells patch-clamp techniques
ISSN号0253-9756
文献子类Article
英文摘要AIM: To determine whether or not the dopamine (DA) can induce ionic current in single neuron acutely dissociated from different central areas including striatum, ventral tegmental area (VTA), substantia nigra pars compacta (SNC) and hippocampal CA(1) area. METHODS: Using a new patch-clamp whole-cell recording technic, namely nystatin-perforated whole-cell configuration under voltage-clamp mode. RESULTS: In 36 single neurons isolated-from the striatum and VTA, DA 10-1000 mu mol . L(-1) was quite diverse to elicit ionic current response. In 19 SNC neurons, 5 neurons (26%) response to 1 mmol . L(-1) DA represented as a small outward current (11.3 +/- 2.4 pA) at a holding potential (V-H) of - 20 mV. In. 25 of 69 (36%) examined hippocampal CA(1) pyramidal neurons, however, application of DA induced 3 types of current responses: outward current (8 neurons) accompanied with an increase of membrane conductance, slow inward current (5 neurons) with an decrease of membrane conductance and outward-following inward current (12 neurons) at a V-H of - 20 mV. The concentration-response relationship of DA-induced currents showed the typical sigmoid shape with the threshold dose, being the maxium response dose are 3 mu mol . L(-1) and 1 mmol . L(-1) respectively. The current-voltage (I-V) relationship of DA-induced responses did not show any voltage-dependent manner and the reversal potential (EDA) was close to the equilibrium potential of potassium (E(K)) calculated with the Nernst equation. TEA 5 mol . L(-1) effectively inhibited the DA-induced response. CONCLUSION: These results suggest that DA-induced outward current is carried by K+ in single hippocampel CA(1) pyramidal neuron.
WOS关键词HIPPOCAMPAL-FORMATION ; CORPUS STRIATUM ; RECEPTORS
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:339684
WOS记录号WOS:A1996TQ30000005
出版者ACTA PHARMACOLOGICA SINICA
源URL[http://119.78.100.183/handle/2S10ELR8/275009]  
专题中国科学院上海药物研究所
作者单位1.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA
2.SUN YAT SEN UNIV MED SCI,DEPT PHYSIOL,CANTON 510089,PEOPLES R CHINA
推荐引用方式
GB/T 7714
Wu, J,Chen, PX,Jin, GZ. Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS[J]. ACTA PHARMACOLOGICA SINICA,1996,17(1):23-&.
APA Wu, J,Chen, PX,&Jin, GZ.(1996).Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS.ACTA PHARMACOLOGICA SINICA,17(1),23-&.
MLA Wu, J,et al."Dopamine-induced ionic currents in acutely dissociated rat neurons of CNS".ACTA PHARMACOLOGICA SINICA 17.1(1996):23-&.

入库方式: OAI收割

来源:上海药物研究所

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