YUANHUACIN-A IS A SELECTIVE ANTAGONIST OF PHORBOL ESTER RECEPTOR IN PROTEIN-KINASE-C
文献类型：期刊论文
| 作者 | ZHANG, DC; CAO, CX; ZHANG, CJ; ZHOU, BN |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 1993-07 |
| 卷号 | 36期号:7页码:803-808 |
| 关键词 | PROTEIN KINASE-C PHORBOL ESTERS YUANHUACIN-A 3H-PHORBOL-12 13-DIBUTYRATE |
| ISSN号 | 1001-652X |
| 文献子类 | Article |
| 英文摘要 | Protein kinase C with a molecular weight of 82 kD has been purified to electrophoresis homogenous from rat brain through a series of chromatography columns including DE-52, Sepharose G-200 and phenyl-Sepharose. The enzyme possessed autophosphorylation activity. Yuanhuacin A inhibited the H-3-phorbol-12, 13-dibutyrate (H-3-PdBu) binding of PKC with an IC50 value of 1.48 +/- 0.28 X 10(-8) mol/L when the concentration of H-3-PdBu was 1.5 X 10(-9) mol/L (K(i) = 1.2 X 10(-8) mol/L). Yuanhuacin A inhibited the PdBu-stimulated PKC activity in the catalysis of the phosphorylation of Histone III-S with an IC50 of 2.82 +/- 0.37 X 10(-9) mol/L (PdBu = 10(-6) mol/L), while it had no effect on the basal and Ca2+-stimulated PKC activity in the same assay system. This result suggests that Yuanhuacin A is a selective antagonist of the phorbol ester receptor in protein kinase C. |
| WOS关键词 | SIGNAL TRANSDUCTION ; INHIBITOR ; CALCIUM ; POTENT |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:A1993LR67000005 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275130]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | ZHANG, DC |
| 作者单位 | ACAD SINICA,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | ZHANG, DC,CAO, CX,ZHANG, CJ,et al. YUANHUACIN-A IS A SELECTIVE ANTAGONIST OF PHORBOL ESTER RECEPTOR IN PROTEIN-KINASE-C[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1993,36(7):803-808. |
| APA | ZHANG, DC,CAO, CX,ZHANG, CJ,&ZHOU, BN.(1993).YUANHUACIN-A IS A SELECTIVE ANTAGONIST OF PHORBOL ESTER RECEPTOR IN PROTEIN-KINASE-C.SCIENCE IN CHINA SERIES B-CHEMISTRY,36(7),803-808. |
| MLA | ZHANG, DC,et al."YUANHUACIN-A IS A SELECTIVE ANTAGONIST OF PHORBOL ESTER RECEPTOR IN PROTEIN-KINASE-C".SCIENCE IN CHINA SERIES B-CHEMISTRY 36.7(1993):803-808. |
入库方式： OAI收割
来源：上海药物研究所
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