EFFECT OF BRAIN-LESIONS ON [H-3] OHMEFENTANYL BINDING-SITE DENSITIES IN THE RAT STRIATUM AND SUBSTANTIA-NIGRA
文献类型：期刊论文
| 作者 | MASUO, Y; WANG, H; PELAPRAT, D; CHI, ZQ ; ROSTENE, W
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| 刊名 | CHEMICAL & PHARMACEUTICAL BULLETIN
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| 出版日期 | 1992-09 |
| 卷号 | 40期号:9页码:2520-2524 |
| 关键词 | [H-3]OHMEFENTANYL [H-3][D-ALA2, MEPHE4, GLY-OL5]ENKEPHALIN ([H-3]DAGO) MU-OPIOID RECEPTOR AUTORADIOGRAPHY BRAIN LESION 6-HYDROXYDOPAMINE QUINOLINIC ACID STRIATUM SUBSTANTIA-NIGRA BINDING SITE |
| ISSN号 | 0009-2363 |
| 文献子类 | Article |
| 英文摘要 | We have recently demonstrated that [H-3]ohmefentanyl, a non-peptidergic opioid ligand which was suggested to cross the blood brain barrier in contrast to other peptidergic opioid ligands, bound not only to mu opioid receptor sites but also to sigma sites. In order to examine whether [H-3]ohmefentanyl can be used as a marker for mu sites, we investigated the effects of brain lesions on [H-3]ohmefentanyl binding site densities, as compared with [H-3][D-Ala2, MePhe4, Gly-ol5]enkephalin ([H-3]DAGO), a selective mu ligand. These binding site densities were measured by quantitative autoradiography in the rat striatum and substantia nigra, two brain structures known to contain a high density of mu receptors, following lesions of the nigro-striatal dopaminergic pathway and striatal intrinsic neurons. Following unilateral nigral lesion with 6-hydroxydopamine, [H-3]ohmefentanyl binding site densities were decreased in the patches (-35%) and matrix (-20%) of the ipsilateral striatum and in the lesioned substantia nigra pars compacta (-49%). Unilateral striatal lesion with quinolinic acid induced 72%, 61% and 50% decreases in [H-3]ohmefentanyl binding in the patches and matrix of the lesioned striatum and in the ipsilateral substantia nigra pars reticulata, respectively. Similar results were obtained in the binding of [H-3]DAGO. Indeed, a significant linear correlation was observed between [H-3]-ohmefentanyl and [H-3]DAGO binding site densities. Therefore, mu opioid receptors may be mainly located on intrinsic neurons in the striatum, dopaminergic cell bodies in the substantia nigra pars compacta and nerve terminals of striatal efferents in the substantia nigra pars reticulata. The present study revealed that [H-3]ohmefentanyl binding sites follow a pattern similar to that observed for mu opioid receptors in response to lesions of the nigro-striatal dopaminergic pathway and striatal intrinsic neurons. Possible binding of [H-3]ohmefentanyl to sigma sties may not influence changes in mu sites caused by such lesions. [H-3]Ohmefentanyl may thus be a useful tool as a marker for mu opioid receptors. |
| WOS关键词 | NEUROTENSIN-CONTAINING NEURONS ; DELTA-OPIOID RECEPTORS ; KAINIC ACID ; QUANTITATIVE AUTORADIOGRAPHY ; H-3 DIHYDROTETRABENAZINE ; OPIATE RECEPTORS ; QUINOLINIC ACID ; IBOTENIC ACID ; MU ; LOCALIZATION |
| WOS研究方向 | Pharmacology & Pharmacy ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:A1992JR95500049 |
| 出版者 | PHARMACEUTICAL SOC JAPAN |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275151]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | MASUO, Y |
| 作者单位 | ACAD SINICA, SHANGHAI INST MAT MED, SHANGHAI 200031, PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | MASUO, Y,WANG, H,PELAPRAT, D,et al. EFFECT OF BRAIN-LESIONS ON [H-3] OHMEFENTANYL BINDING-SITE DENSITIES IN THE RAT STRIATUM AND SUBSTANTIA-NIGRA[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,1992,40(9):2520-2524. |
| APA | MASUO, Y,WANG, H,PELAPRAT, D,CHI, ZQ,&ROSTENE, W.(1992).EFFECT OF BRAIN-LESIONS ON [H-3] OHMEFENTANYL BINDING-SITE DENSITIES IN THE RAT STRIATUM AND SUBSTANTIA-NIGRA.CHEMICAL & PHARMACEUTICAL BULLETIN,40(9),2520-2524. |
| MLA | MASUO, Y,et al."EFFECT OF BRAIN-LESIONS ON [H-3] OHMEFENTANYL BINDING-SITE DENSITIES IN THE RAT STRIATUM AND SUBSTANTIA-NIGRA".CHEMICAL & PHARMACEUTICAL BULLETIN 40.9(1992):2520-2524. |
入库方式： OAI收割
来源：上海药物研究所
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