A DNA HYBRIDIZATION ASSAY FOR USE IN DRUG SENSITIVITY TESTS INVITRO FOR PLASMODIUM-FALCIPARUM UNDER FIELD CONDITIONS
文献类型：期刊论文
| 作者 | WAKI, S; NAKANE, K; NAKAMURA, N; ZHU, MY; DAI, ZR; CHEN, L; TANABE, K |
| 刊名 | TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
 |
| 出版日期 | 1992-05 |
| 卷号 | 86期号:3页码:227-228 |
| ISSN号 | 0035-9203 |
| DOI | 10.1016/0035-9203(92)90283-I |
| 文献子类 | Article |
| 英文摘要 | A deoxyribonucleic acid (DNA) hybridization technique was applied to in vitro drug sensitivity testing of P. falciparum using a synthetic 21-mer oligonucleotide coupled to alkaline phosphatase (PFR1-AP) to monitor development of parasite stages in culture. The density of the coloured spot clearly distinguished schizonts from ring forms. This assay system was applied in the field on Hainan Island, China. Blood samples obtained from patients were cultivated in the presence of antimalarial drugs and the minimum drug concentration required to inhibit development of parasites was determined by the DNA hybridization assay and by microscopical observation of Giemsa-stained blood smears. The 2 methods yielded identical results, indicating that the DNA hybridization assay can be used for in vitro drug sensitivity testing under field conditions. |
| WOS研究方向 | Public, Environmental & Occupational Health ; Tropical Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:A1992JD30400002 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275166]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | WAKI, S |
| 作者单位 | 1.CHINESE ACAD SCI, SHANGHAI INST MATERIA MED, SHANGHAI 200031, PEOPLES R CHINA 2.SECOND MIL MED UNIV, SHANGHAI 200433, PEOPLES R CHINA 3.OSAKA INST TECHNOL, BIOL LAB, OSAKA 535, JAPAN |
| 推荐引用方式 GB/T 7714 | WAKI, S,NAKANE, K,NAKAMURA, N,et al. A DNA HYBRIDIZATION ASSAY FOR USE IN DRUG SENSITIVITY TESTS INVITRO FOR PLASMODIUM-FALCIPARUM UNDER FIELD CONDITIONS[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE,1992,86(3):227-228. |
| APA | WAKI, S.,NAKANE, K.,NAKAMURA, N.,ZHU, MY.,DAI, ZR.,...&TANABE, K.(1992).A DNA HYBRIDIZATION ASSAY FOR USE IN DRUG SENSITIVITY TESTS INVITRO FOR PLASMODIUM-FALCIPARUM UNDER FIELD CONDITIONS.TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE,86(3),227-228. |
| MLA | WAKI, S,et al."A DNA HYBRIDIZATION ASSAY FOR USE IN DRUG SENSITIVITY TESTS INVITRO FOR PLASMODIUM-FALCIPARUM UNDER FIELD CONDITIONS".TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE 86.3(1992):227-228. |
入库方式： OAI收割
来源：上海药物研究所
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