EFFECTS OF LYCOBETAINE ON CHROMATIN STRUCTURE AND ACTIVITY OF MURINE HEPATOMA-CELLS
文献类型：期刊论文
| 作者 | LIU, J ; YANG, SL; XU, B
|
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES
 |
| 出版日期 | 1990-12 |
| 卷号 | 33期号:12页码:1459-1465 |
| 关键词 | LYCOBETAINE CHROMATIN ONCOGENE |
| ISSN号 | 1001-652X |
| 文献子类 | Article |
| 英文摘要 | The effects of lycobetaine (LBT) on DNA single strand break and chromatin conformation were examined by in-situ nick translation method. It was found that LBT did not cause DNA single strand break. After 2-h incubation of murine hepatoma cells with 1-50 mu-g/m1 LBT in vitro, the chromatin transcription activity was inhibited gradually. This effect was time-and dose-dependent. Actinomycin D produced a similar effect; 10-hydroxy camptothecin not only caused DNA single strand break, but also altered chromatin conformation; homoharringtonine had no marked influence on either. By molecular hybridization technique, it was found that the effect of LBT on individual genes was somewhat different. After 2-h incubation of the cells with LBT, the sensitivities of c-myc, N-ras, and beta-2-microglobulin genes to DNase I were decreased from 75 +/- 6, 66 +/- 4, 70 +/- 8% to 28 +/- 8, 25 +/- 5, 28 +/- 7%, respectively, while that of c-myb and beta-globin genes (8 + 6%, 6 + 5%) did not change obviously. |
| WOS关键词 | DNA |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:A1990EP91400006 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275230]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | LIU, J |
| 作者单位 | CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA. |
| 推荐引用方式 GB/T 7714 | LIU, J,YANG, SL,XU, B. EFFECTS OF LYCOBETAINE ON CHROMATIN STRUCTURE AND ACTIVITY OF MURINE HEPATOMA-CELLS[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES,1990,33(12):1459-1465. |
| APA | LIU, J,YANG, SL,&XU, B.(1990).EFFECTS OF LYCOBETAINE ON CHROMATIN STRUCTURE AND ACTIVITY OF MURINE HEPATOMA-CELLS.SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES,33(12),1459-1465. |
| MLA | LIU, J,et al."EFFECTS OF LYCOBETAINE ON CHROMATIN STRUCTURE AND ACTIVITY OF MURINE HEPATOMA-CELLS".SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES 33.12(1990):1459-1465. |
入库方式： OAI收割
来源：上海药物研究所
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