Adenosine A(1) receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury
文献类型：期刊论文
| 作者 | Winerdal, Max2; Winerdal, Malin E.3; Wang, Ying-Qing4,5 ; Fredholm, Bertil B.4; Winqvist, Ola1,3; Aden, Ulrika1,2
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| 刊名 | PURINERGIC SIGNALLING
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| 出版日期 | 2016-03 |
| 卷号 | 12期号:1页码:89-101 |
| 关键词 | Brain hypoxic-ischemia Neuroimmunomodulation Neonatology Adenosine A(1) receptor Cellular immunity Statistical data interpretation |
| ISSN号 | 1573-9538 |
| DOI | 10.1007/s11302-015-9482-3 |
| 文献子类 | Article |
| 英文摘要 | Neonatal brain hypoxic ischemia (HI) often results in long-term motor and cognitive impairments. Post-ischemic inflammation greatly effects outcome and adenosine receptor signaling modulates both HI and immune cell function. Here, we investigated the influence of adenosine A(1) receptor deficiency (A(1)R(-/-)) on key immune cell populations in a neonatal brain HI model. Ten-day-old mice were subjected to HI. Functional outcome was assessed by open locomotion and beam walking test and infarction size evaluated. Flow cytometry was performed on brain-infiltrating cells, and semi-automated analysis of flow cytometric data was applied. A(1)R(-/-) mice displayed larger infarctions (+33 %, p < 0.05) and performed worse in beam walking tests (44 % more mistakes, p < 0.05) than wild-type (WT) mice. Myeloid cell activation after injury was enhanced in A(1)R(-/-) versus WT brains. Activated B lymphocytes expressing IL-10 infiltrated the brain after HI in WT, but were less activated and did not increase in relative frequency in A(1)R(-/-). Also, A(1)R(-/-) B lymphocytes expressed less IL-10 than their WT counterparts, the A(1)R antagonist DPCPX decreased IL-10 expression whereas the A(1)R agonist CPA increased it. CD4(+) T lymphocytes including FoxP3(+) T regulatory cells, were unaffected by genotype, whereas CD8(+) T lymphocyte responses were smaller in A(1)R(-/-) mice. Using PCA to characterize the immune profile, we could discriminate the A(1)R(-/-) and WT genotypes as well as sham operated from HI-subjected animals. We conclude that A(1)R signaling modulates IL-10 expression by immune cells, influences the activation of these cells in vivo, and affects outcome after HI. |
| WOS关键词 | FOCAL CEREBRAL-ISCHEMIA ; FLOW-CYTOMETRY ; B-CELLS ; INTERNATIONAL UNION ; MICE ; DAMAGE ; CLASSIFICATION ; LYMPHOCYTES ; EXPRESSION ; RAT |
| 资助项目 | Swedish Medical Research Council[2011-3981] ; Swedish Medical Research Council[2553] ; Stockholm County Council[ALF 20120450] ; Karolinska Institutet[ALF 20120450] ; Marianne and Marcus Wallenberg Foundation[MMW 2011.0085] ; Swedish Brain Foundation[FO2013-0073] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000370074400006 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276130]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Winerdal, Malin E. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China; 2.Karolinska Inst, Dept Womens & Childrens Hlth, S-17176 Stockholm, Sweden; 3.Karolinska Inst, Dept Med, S-17176 Stockholm, Sweden; 4.Karolinska Inst, Dept Physiol & Pharmacol, S-17176 Stockholm, Sweden; 5.Karolinska Univ Sjukhuset, Neonatal Res Unit Q2 07, S-17176 Stockholm, Sweden |
| 推荐引用方式 GB/T 7714 | Winerdal, Max,Winerdal, Malin E.,Wang, Ying-Qing,et al. Adenosine A(1) receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury[J]. PURINERGIC SIGNALLING,2016,12(1):89-101. |
| APA | Winerdal, Max,Winerdal, Malin E.,Wang, Ying-Qing,Fredholm, Bertil B.,Winqvist, Ola,&Aden, Ulrika.(2016).Adenosine A(1) receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury.PURINERGIC SIGNALLING,12(1),89-101. |
| MLA | Winerdal, Max,et al."Adenosine A(1) receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury".PURINERGIC SIGNALLING 12.1(2016):89-101. |
入库方式： OAI收割
来源：上海药物研究所
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