Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides
文献类型:期刊论文
作者 | Fu, Yunbin2,3; Cheng, Likun2; Meng, Yanyu1,2; Li, Shuguang2; Zhao, Xiaoming2; Du, Yuguang2,4; Yin, Heng2 |
刊名 | CARBOHYDRATE POLYMERS
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出版日期 | 2015-12-10 |
卷号 | 134页码:740-744 |
关键词 | Cellulosimicrobium cellulans Enzymatic degradation Curdlan High-set gel Alkali-neutralization treatment (1 -> 3)-Linked beta-D-glucan oligosaccharides |
ISSN号 | 0144-8617 |
DOI | 10.1016/j.carbpol.2015.08.019 |
文献子类 | Article |
英文摘要 | In order to find an efficient enzymatic tool for curdlan degradation to produce (1 -> 3)-linked beta-D-glucan oligosaccharides, strain E4-5 (registration number JN089883, Genbank) was isolated from seaside soil. The 16S rRNA gene sequencing classified it as Cellulosimicrobium cellulans. It was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. The ferments of strain E4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. Due to the products with less amount of glucose, it was assumed that endo-1,3-beta-glucanases of strain E4-5 had a greater hydrolyzing effect than exo-1,3-beta-glucanases. This indicated that strain E4-5 was a promising microorganism to hydrolyze (1 -> 3)-linked beta-D-glucan. Moreover, alkali-neutralization pretreatment was effective for promoting a more diversified degree of polymerization (DP) of (1 -> 3)-linked beta-D-glucan oligosaccharides under enzymatic hydrolysis and will pave the way for making full use of curdlan for production of glucan oligosaccharides. (C) 2015 Elsevier Ltd. All rights reserved. |
WOS关键词 | ALPHA-AMYLASE ; RESISTANCE ; GELATION |
资助项目 | Hi-Tech Research and Development Program of China[2011AA10A205] ; Hi-Tech Research and Development Program of China[2012AA021501] ; National Natural Science Foundation of China[31370811] |
WOS研究方向 | Chemistry ; Polymer Science |
语种 | 英语 |
WOS记录号 | WOS:000364255800089 |
出版者 | ELSEVIER SCI LTD |
源URL | [http://119.78.100.183/handle/2S10ELR8/276285] ![]() |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Du, Yuguang |
作者单位 | 1.Henan Agr Univ, Coll Sci, Zhengzhou 450002, Henan, Peoples R China; 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Grp 1805, Dalian 116023, Liaoning, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Fu, Yunbin,Cheng, Likun,Meng, Yanyu,et al. Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides[J]. CARBOHYDRATE POLYMERS,2015,134:740-744. |
APA | Fu, Yunbin.,Cheng, Likun.,Meng, Yanyu.,Li, Shuguang.,Zhao, Xiaoming.,...&Yin, Heng.(2015).Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides.CARBOHYDRATE POLYMERS,134,740-744. |
MLA | Fu, Yunbin,et al."Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides".CARBOHYDRATE POLYMERS 134(2015):740-744. |
入库方式: OAI收割
来源:上海药物研究所
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