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Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro

文献类型：期刊论文

作者Chen, Qixin2; Mei, Xifan3; Han, Guanying1,3,4; Ling, Peixue1; Guo, Bin3; Guo, Yuewei4; Shao, Huarong1; Wang, Guan2; Cui, Zan3; Bai, Yuxin2
刊名CARBOHYDRATE POLYMERS
出版日期2015-10-20
卷号131页码:363-369
关键词Xanthan gum Sodium nitroprusside Rabbit chondrocytes Apoptosis
ISSN号0144-8617
DOI10.1016/j.carbpol.2015.06.004
文献子类Article
英文摘要We have previously reported that intra-articular injection of xanthan gum (XG) could significantly ameliorate the degree of joint cartilage degradation and pain in experimental osteoarthritis (OA) model in vivo. In this present study, we evaluated the protective effect of XG against Sodium nitroprusside (SNP)induced rabbit articular chondrocytes apoptosis in vitro. Rabbit articular chondrocytes were incubated with various concentrations of XG for 24 h prior to 0.5 mmol/L SNP co-treatment for 24 h. The proliferation of chondrocytes was analyzed using MU assay. The chondrocytes early apoptosis rates were evaluated using Annexin V-FITC/PI flow cytometry. The morphology of apoptosis chondrocytes were observed by scanning electron microscopy (SEM). The loss/disruption of mitochondrial membrane potential was detected using rhodamin 123 by confocal microscope. The concentration of prostaglandin E-2 (PGE(2)) in cell culture supernatants was evaluated using ELISA assay. The results showed that XG could significantly reverse SNP-reduced cell proliferation and inhibited cell early apoptosis rate in a dose-dependent manner. XG alleviated loss/disruption of mitochondrial membrane potential and decreased the PGE2 level of chondrocytes cell culture supernatants in SNP-induced chondrocytes. These results of the present research strongly suggest that XG can protect rabbit articular chondrocytes against SNP-induced apoptosis in vitro. (C) 2015 Elsevier Ltd. All rights reserved.
WOS关键词OXIDE-INDUCED APOPTOSIS ; NITRIC-OXIDE ; MITOCHONDRIAL DYSFUNCTION ; CARTILAGE DEGRADATION ; OSTEOARTHRITIS ; INJECTION ; RECOVERY ; THERAPY ; DISEASE ; MODEL
资助项目National Major Scientific and Technological Special Project for "Significant New Drugs Development" during the Twelfth Five-year Plan Period[2012ZX093016-002] ; National Natural Science Foundation of China[81302681] ; Natural Science Foundation of Liaoning Province[2013022051] ; Aohongboze Foundation, the President Fund of Liaoning Medical University[XZJJ20130106-02] ; Quanmin Oral Graduate Sci-tech Innovation Foundation[00000000] ; President Fund of Liaoning Medical University[QM2014007]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000361183600043
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/276357]  
专题中国科学院上海药物研究所
通讯作者Han, Guanying
作者单位1.Inst Biopharmaceut Shandong Prov, Postdoctoral Sci Res Workstat, Jinan 250101, Peoples R China;
2.Liaoning Med Univ, Sch Pharmaceut Sci, Jinzhou 121001, Peoples R China;
3.Liaoning Med Univ, Affiliated Hosp 1, Jinzhou 121001, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Qixin,Mei, Xifan,Han, Guanying,et al. Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro[J]. CARBOHYDRATE POLYMERS,2015,131:363-369.
APA Chen, Qixin.,Mei, Xifan.,Han, Guanying.,Ling, Peixue.,Guo, Bin.,...&Xu, Fang.(2015).Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro.CARBOHYDRATE POLYMERS,131,363-369.
MLA Chen, Qixin,et al."Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro".CARBOHYDRATE POLYMERS 131(2015):363-369.

入库方式： OAI收割

来源：上海药物研究所

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