中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma

文献类型:期刊论文

作者Zhang, Shu2; Yang, Xiaojing1,3; Shi, Hui4; Li, Mei1; Xue, Qun2; Ren, Hanru2; Yao, Li3; Chen, Xueyu5; Zhang, Jianguo2; Wang, Huijie6
刊名JOURNAL OF MOLECULAR HISTOLOGY
出版日期2014-06
卷号45期号:3页码:283-292
ISSN号1567-2379
关键词Esophageal squamous cell carcinoma LAP3 Tumor immunology Proliferation Migration
DOI10.1007/s10735-014-9566-3
文献子类Article
英文摘要Leucine aminopeptidases (LAPs) were associated with tumor cell proliferation, invasion and/or angiogenesis. We aimed to examine the biological function of LAP3 in esophageal squamous cell carcinoma (ESCC). LAP3 expressions were examined in human ESCC tissue and cell lines ECA109 and TE1 cells. Recombinant pSilencer4.1-LAP3-shRNA was transfected into ECA109 cells to silence LAP3 expression. The effects of LAP3 silencing on ECA109 cell proliferation in vitro were evaluated. Flow cytometry profiling was used to detect the differentiate cell cycle distribution in LAP3-silenced ECA109 cells. Wound-healing assay and transwell assay were used to examine the activities of migration and invasion in LAP3-silenced ECA109 cells. We overexpressed LAP3 in TE1 cells to find out the corresponding results. LAP3 expression level was abundance in ESCC tissue. LAP3 silencing significantly reduced ECA109 cell proliferation and colony formation. The knockdown of LAP3 resulted in cell cycle arrest at G1-phase. Moreover, over expression of LAP3 favors TE1 cell proliferation and invasiveness which also confirms its contribution in malignant development. We came to the conclusion that LAP3 contributed to ESCC progression by overcoming cell cycle arrest. The proliferative and migration effects of LAP3 might contribute to malignant development of human ESCC.
WOS关键词CANCER-CELLS ; ANGIOTENSIN-II ; EXPRESSION ; PROLIFERATION ; ANGIOGENESIS ; ENZYMOLOGY
WOS研究方向Cell Biology
语种英语
出版者SPRINGER
WOS记录号WOS:000334439500005
源URL[http://119.78.100.183/handle/2S10ELR8/277063]  
专题中国科学院上海药物研究所
通讯作者Zhang, Jianguo
作者单位1.Nantong Univ, Affiliated Hosp, Dept Med Oncol, Nantong 226001, Jiangsu, Peoples R China;
2.Nantong Univ, Affiliated Hosp, Dept Pathol, Nantong 226001, Jiangsu, Peoples R China;
3.Nantong Univ, Coll Med, Dept Immunol, Nantong 226001, Jiangsu, Peoples R China;
4.Nantong Univ, Affiliated Hosp, Dept Thorac Surg, Nantong 226001, Jiangsu, Peoples R China;
5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
6.Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai 200032, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Shu,Yang, Xiaojing,Shi, Hui,et al. Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma[J]. JOURNAL OF MOLECULAR HISTOLOGY,2014,45(3):283-292.
APA Zhang, Shu.,Yang, Xiaojing.,Shi, Hui.,Li, Mei.,Xue, Qun.,...&Wang, Huijie.(2014).Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma.JOURNAL OF MOLECULAR HISTOLOGY,45(3),283-292.
MLA Zhang, Shu,et al."Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma".JOURNAL OF MOLECULAR HISTOLOGY 45.3(2014):283-292.

入库方式: OAI收割

来源:上海药物研究所

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