BuShenYiQi Formula strengthens Thl response and suppresses Th2-Th17 responses in RSV-induced asthma exacerbated mice
文献类型：期刊论文
| 作者 | Wang, Jia2,3 ; Wu, Jinfeng2,3; Kong, Lingwen2,3; Nurahmat, Muhammat1,2,4; Chen, Meixia1,2,3; Luo, Qingli2,3; Li, Bei2,3; Wu, Xiao2,3; Dong, Jingcheng2,3
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2014-05-28 |
| 卷号 | 154期号:1页码:131-147 |
| 关键词 | BuShenYiQi Formula Asthma exacerbation Respiratory syncytial virus Allergic airway inflammation Helper T lymphocyte |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2014.03.041 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: The prevalence of allergic asthma has been increased rapidly in recent years. About 20% of all these sufferers have experienced asthma exacerbation. Although corticosteroids and P-agonists therapy improves serious asthma symptoms, they can't completely cure these allergic diseases. BuShenYiQi Formula (BSYQF) has been widely used to treat bronchial asthma and its exacerbation for decades in Huashan Hospital of Fudan University, China. Nevertheless, the mechanisms of BSYQF' anti-asthmatic effects haven't been fully elucidated. In this study, we evaluated the involvement of Thl, Th2 and Th17 cells in the anti-asthmatic effects of BSYQF in Respiratory Syncytial Virus (RSV)-induced asthma exacerbated mice. Materials and methods: BALB/c mice were challenged with ovalbumin (OVA), followed by RSV infections for establishment of asthma exacerbated model. Airway hyperresponsiveness (AHR) was examined by direct airway resistance analysis. Bronchoalveolar lavage fluid (BALE) was assessed for inflammatory cell counts and secreted levels of cytokines. Lung tissues were detected for inflammatory cell infiltration and mucus hypersecretion. Subsequently, CD4(+)FT cells and alveolar macrophages were sorted and purified from mice lungs in different groups. CD4(+)T cell subpopulations including the expression levels of important transcription factors in T lymphocyte polarization were examined. In asthma exacerbation group, the purified CD4(+)T cells and macrophages were co-cultured, and the changes of co-cultured cells with BSYQF treatment were further analyzed in vitro. Results: BSYQF significantly attenuated airway hyperresponsiveness and inhibited inflammatory cell infiltration, especially for excessive infiltration of eosinophils and neutrophils. Histopathological analysis showed that BSYQF could suppress airway inflammation and RSV replication. The decreases of antigenspecific IgE, IL-4, IL-5, IL-6, IL-17a and increases of IFN-gamma, IL-12 were observed in BALF, lung homogenate or serum after BSYQF treatment. We further confirmed that BSYQF could down-regulate Th2-Th17 cell proportions with lower expressions of GATA3, STAT6 and ROR gamma T, and up-regulate Thl cell proportion with higher expression of T-bet. And as a result of strengthened Thl-response, activated macrophages were also observed by remarkable enhancement of signature gene expressions and phagocytosis. Conclusions: BSYQF can significantly attenuate RSV-induced asthma exacerbation. These effects may be mediated at least partially by regulating the balance between Thl and Th2-Th17 responses. (c) 2014 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | REGULATORY T-CELLS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; AIRWAY HYPERRESPONSIVENESS ; IN-VIVO ; HERBAL INTERVENTIONS ; IMMUNE PATHWAY ; VIRUS ; ALLERGY ; INFLAMMATION ; INFECTION |
| 资助项目 | National Natural Science Foundation of China[81173390/H2902] ; National Basic Science Program of China[2009CB523000] ; Shanghai Science and Technology Committee[09XD1400700] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000336952600011 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277066]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Dong, Jingcheng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Fudan Univ, Huashan Hosp, Dept Integrat Med, Shanghai 200040, Peoples R China; 3.Fudan Univ, Inst Integrated Tradit Chinese & Western Med, Shanghai 200040, Peoples R China; 4.Xinjiang Uygur Med Coll, Xinjiang 848000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jia,Wu, Jinfeng,Kong, Lingwen,et al. BuShenYiQi Formula strengthens Thl response and suppresses Th2-Th17 responses in RSV-induced asthma exacerbated mice[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,154(1):131-147. |
| APA | Wang, Jia.,Wu, Jinfeng.,Kong, Lingwen.,Nurahmat, Muhammat.,Chen, Meixia.,...&Dong, Jingcheng.(2014).BuShenYiQi Formula strengthens Thl response and suppresses Th2-Th17 responses in RSV-induced asthma exacerbated mice.JOURNAL OF ETHNOPHARMACOLOGY,154(1),131-147. |
| MLA | Wang, Jia,et al."BuShenYiQi Formula strengthens Thl response and suppresses Th2-Th17 responses in RSV-induced asthma exacerbated mice".JOURNAL OF ETHNOPHARMACOLOGY 154.1(2014):131-147. |
入库方式： OAI收割
来源：上海药物研究所
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