N-alkylpyridinium quaternization for assisting electrospray ionization of sterols in oil by quadrupole-time of flight mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Hang1,2; Wang, Haoyang1; Zhang, Li1; Zhang, Jing1; Leng, Jiapeng1; Cai, Tingting1; Guo, Yinlong1 |
| 刊名 | JOURNAL OF MASS SPECTROMETRY
 |
| 出版日期 | 2013-10 |
| 卷号 | 48期号:10页码:1101-1108 |
| 关键词 | illegal cooking oil isotope-coded reagent pyridinium quaternization chemical derivatization quadrupole-time of flight mass spectrometry |
| ISSN号 | 1076-5174 |
| DOI | 10.1002/jms.3265 |
| 文献子类 | Article |
| 英文摘要 | Rationale: The illegal cooking oil has become a serious social problem and raised widespread alarm recently. However, an efficient and sensitive technique for identifying the potential illegal cooking oil is still unavailable, especially when mixed with the ordinary ones; there is an urgent need to develop an efficient method for identifying the illegal cooking oil. Sterols in the cooking oil could be used as an indicator to identify the source and quality of oil by detecting the kinds of phytosterols and zoosterols. However, those sterols are difficult to be ionized by electrospray ionization, which resulted in the low sensitivity in electrospray ionization (ESI)-mass spectrometric (MS) analysis. Methods: N-alkylpyridinium isotope quaternization was extended to charge label sterols in different cooking oil and attached N-cationic pyridinium tag onto the sterols in the presence of trifluoromethanesulfonic anhydride (Tf2O); the kinds of sterols were identified and quantified by comparing d(0)/d(5) pairs and product scan from ESI-quadrupole-time of flight (Q-TOF) MS analysis. Results: The derivatized sterols were attached with permanent charge, resulting in the significant enhancement of ionization in ESI-Q-TOF MS analysis. The detection limits of analytes were improved to 0.02-0.05 ng/mL; different kinds of phytosterol, zoosterol and oxides were identified and quantified by comparing d(0)/d(5) pairs from full scan and product scan. The method was applied in the detection of zoosterol for identifying the potential recycled cooking oil, even when the illegal oil has been blended into the ordinary one. More zoosterol was detected in the recycled oil compared with other cooking oil. Conclusions: The use of N-alkylpyridinium isotope quaternization method provided an alternative method for identifying the potential illegal cooking oil. Copyright (C) 2013 John Wiley & Sons, Ltd. Supporting information may be found in the online version of this article. |
| WOS关键词 | VEGETABLE-OILS ; OLIVE OIL ; LIQUID-CHROMATOGRAPHY ; ISOTOPE QUATERNIZATION ; QUANTITATIVE-ANALYSIS ; SPECTROSCOPIC METHODS ; OXIDATION-PRODUCTS ; BETA-SITOSTEROL ; EDIBLE OILS ; HPLC-MS |
| 资助项目 | National Natural Science Foundation of China[20902104] ; National Natural Science Foundation of China[21072215] ; National Natural Science Foundation of China[21172250] ; Innovation Method Fund of China[2010IM030900] ; CAS[YZ200938] ; CAS[YG2010056] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000339171200002 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277428]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, Yinlong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai Mass Spectrometry Ctr, Shanghai 200032, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hang,Wang, Haoyang,Zhang, Li,et al. N-alkylpyridinium quaternization for assisting electrospray ionization of sterols in oil by quadrupole-time of flight mass spectrometry[J]. JOURNAL OF MASS SPECTROMETRY,2013,48(10):1101-1108. |
| APA | Wang, Hang.,Wang, Haoyang.,Zhang, Li.,Zhang, Jing.,Leng, Jiapeng.,...&Guo, Yinlong.(2013).N-alkylpyridinium quaternization for assisting electrospray ionization of sterols in oil by quadrupole-time of flight mass spectrometry.JOURNAL OF MASS SPECTROMETRY,48(10),1101-1108. |
| MLA | Wang, Hang,et al."N-alkylpyridinium quaternization for assisting electrospray ionization of sterols in oil by quadrupole-time of flight mass spectrometry".JOURNAL OF MASS SPECTROMETRY 48.10(2013):1101-1108. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。