Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKK beta
文献类型：期刊论文
| 作者 | Iseli, Tristan J.2; Turner, Nigel2; Zeng, Xiao-Yi1,2,3; Cooney, Gregory J.2; Kraegen, Edward W.2; Yao, Sheng4 ; Ye, Yang4; James, David E.2; Ye, Ji-Ming1,2,3,4
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-04-25 |
| 卷号 | 8期号:4 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0062309 |
| 文献子类 | Article |
| 英文摘要 | We recently showed that bitter melon-derived triterpenoids (BMTs) activate AMPK and increase GLUT4 translocation to the plasma membrane in vitro, and improve glucose disposal in insulin resistant models in vivo. Here we interrogated the mechanism by which these novel compounds activate AMPK, a leading anti-diabetic drug target. BMTs did not activate AMPK directly in an allosteric manner as AMP or the Abbott compound (A-769662) does, nor did they activate AMPK by inhibiting cellular respiration like many commonly used anti-diabetic medications. BMTs increased AMPK activity in both L6 myotubes and LKB1-deficient HeLa cells by 20-35%. Incubation with the CaMKK beta inhibitor, STO-609, completely attenuated this effect suggesting a key role for CaMKK beta in this activation. Incubation of L6 myotubes with the calcium chelator EGTA-AM did not alter this activation suggesting that the BMT-dependent activation was Ca2+-independent. We therefore propose that CaMKK beta is a key upstream kinase for BMT-induced activation of AMPK. |
| WOS关键词 | KINASE KINASE-BETA ; DEPENDENT PROTEIN-KINASE ; MOMORDICA-CHARANTIA ; METABOLIC SYNDROME ; COMPLEX ; BERBERINE ; MECHANISM ; RECEPTOR ; IDENTIFICATION ; LOCALIZATION |
| 资助项目 | National Health and Medical Research Council of Australia (NHMRC)[535930] ; Australia-China Special Fund[CH0812110] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000318341400049 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277647]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ye, Ji-Ming |
| 作者单位 | 1.RMIT Univ, Sch Hlth Sci, Melbourne, Vic, Australia; 2.Garvan Inst Med Res, Diabet & Obes Res Program, Sydney, NSW, Australia; 3.RMIT Univ, Hlth Innovat Res Inst, Melbourne, Vic, Australia; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Iseli, Tristan J.,Turner, Nigel,Zeng, Xiao-Yi,et al. Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKK beta[J]. PLOS ONE,2013,8(4). |
| APA | Iseli, Tristan J..,Turner, Nigel.,Zeng, Xiao-Yi.,Cooney, Gregory J..,Kraegen, Edward W..,...&Ye, Ji-Ming.(2013).Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKK beta.PLOS ONE,8(4). |
| MLA | Iseli, Tristan J.,et al."Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKK beta".PLOS ONE 8.4(2013). |
入库方式： OAI收割
来源：上海药物研究所
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