SENP2 regulates MEF2A de-SUMOylation in an activity dependent manner
文献类型：期刊论文
| 作者 | Lu, Han2; Liu, Bin3; You, Shengwu2; Chen, Lei2; Qu Dongmei2; Gu, Minjie2; Lu, Yan1; Chen, Yingyi4; Zhang, Fujun2; Yu, Buwei2 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2013-03 |
| 卷号 | 40期号:3页码:2485-2490 |
| 关键词 | MEF2A SENP2 SUMOylation Activity-dependent stimuli |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-012-2329-x |
| 文献子类 | Article |
| 英文摘要 | SUMOylation has been shown to exert non-negligible influence on transcriptional factors and regulate genes expression by attuning their transcriptional activity. Myocyte-specific enhancer factor-2(MEF2) is a family of transcription factor and play a critical role in embryonic development. The transcriptional activity of MEF2A is highly repressed by SUMOylation in an activity-dependent manner. However, the enzyme that poses de-SUMOylation activity towards MEF2A is still not identified. Here we reported that SENP2 was identified as the major transcriptional dominator and de-SUMOylation enzyme of MEF2A by combining both unbiased shRNA screen and in vivo SUMOylation assays. The SUMOylated form of MEF2A was readily detectable in either SENP2 knockdown cells or knockout embryos. SENP2 markedly enhanced the transcription of MEF2A through directly de-SUMOylation. Moreover, SENP2 protein was accumulated in response to activity-dependent stimuli which in turn mediated activity dependent-regulation of MEF2A de-SUMOylation. Our data clearly show that SENP2 plays an important role in determining the dynamics and functional outcome of MEF2A SUMOylation and transcriptional activation. |
| WOS关键词 | SUMO-SPECIFIC PROTEASES ; DIFFERENTIATION ; EXPRESSION ; TRANSCRIPTION ; SYSTEM ; GENES |
| 资助项目 | National Natural Science Foundation, Beijing, People's Republic of China[81102513] ; National Natural Science Foundation, Beijing, People's Republic of China[81072703] ; Shanghai Health Bureau Scientific Research Foundation, Shanghai, People's Republic of China[10411951700] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000314535600046 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277721]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yu, Buwei |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Clin Ctr Endocrine & Metab Dis, Shanghai Key Lab Endocrine Tumor,Sch Med, Shanghai Inst Endocrinol & Metab,Rui Jin Hosp, Shanghai 200025, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Anesthesiol, Shanghai 200025, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 4.Shanghai Jiao Tong Univ, Key Lab Cell Differentiat & Apoptosis, Dept Pathophysiol, Natl Minist Educ,Sch Med,Rui Jin Hosp, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Han,Liu, Bin,You, Shengwu,et al. SENP2 regulates MEF2A de-SUMOylation in an activity dependent manner[J]. MOLECULAR BIOLOGY REPORTS,2013,40(3):2485-2490. |
| APA | Lu, Han.,Liu, Bin.,You, Shengwu.,Chen, Lei.,Qu Dongmei.,...&Yu, Buwei.(2013).SENP2 regulates MEF2A de-SUMOylation in an activity dependent manner.MOLECULAR BIOLOGY REPORTS,40(3),2485-2490. |
| MLA | Lu, Han,et al."SENP2 regulates MEF2A de-SUMOylation in an activity dependent manner".MOLECULAR BIOLOGY REPORTS 40.3(2013):2485-2490. |
入库方式： OAI收割
来源：上海药物研究所
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