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HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression

文献类型：期刊论文

作者Shi, Chen-Ye2; Fan, Yue1; Liu, Bin3; Lou, Wen-Hui2
刊名CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
出版日期2013
卷号32期号:3页码:561-568
ISSN号1015-8987
关键词Hypoxia HIF-1 QSOX1 Matrix Metalloproteinase Pancreatic Cancer Cell
DOI10.1159/000354460
文献子类Article
英文摘要Background: Quiescin sulfhydryl oxidase 1 (QSOX1), which oxidizes sulfhydryl groups to form disulfide bonds in proteins, is found to be over-expressed in various pancreatic cancer cell lines and patients. QSOX1 promotes invasion of pancreatic cancer cells by activating MMP-2 and MMP-9. However, its regulatory mechanism remains largely undefined. Methods: Realtime PCR and Western blot were employed to detect the expression of QSOX1 in human pancreatic cancer cell lines under hypoxic condition. Luciferase reporter and ChIP assays were used to assess the regulation of QSOX1 by hypoxia-inducible factor 1 (HIF-1). Small interfering RNA (siRNA) was applied to knock down endogenous expression of QSOX1. Matrigel-coated invasion chamber essays were conducted to detect the invasion capacity of QSOX1-depleted cells. Results: Both hypoxia and hypoxia mimicking reagent up-regulated the expression of QSOX1 in human pancreatic cancer cell lines. Knockdown of HIF-1 alpha eliminated hypoxia induced QSOX1 expression. HIF-1a was found directly bound to two hypoxia-response elements (HRE) of QSOX1 gene, both of which were required for HIF-1 induced QSOX1 expression. Moreover, QSOX1 silencing blocked hypoxia-induced pancreatic cancer cells invasion. Conclusion: QSOX1 is a direct target of HIF-1 and may contribute to hypoxia-induced pancreatic cancer cells invasion.Copyright (C) 2013 S. Karger AG, Basel
WOS关键词QUIESCIN-SULFHYDRYL OXIDASE ; THERAPY
WOS研究方向Cell Biology ; Physiology
语种英语
出版者KARGER
WOS记录号WOS:000325415900007
源URL[http://119.78.100.183/handle/2S10ELR8/277787]  
专题中国科学院上海药物研究所
通讯作者Lou, Wen-Hui
作者单位1.Fudan Univ, Zhongshan Hosp, Dept Integrated TCM & Western Med, Shanghai 200032, Peoples R China;
2.Fudan Univ, Zhongshan Hosp, Dept Gen Surg, Shanghai 200032, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Shi, Chen-Ye,Fan, Yue,Liu, Bin,et al. HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2013,32(3):561-568.
APA Shi, Chen-Ye,Fan, Yue,Liu, Bin,&Lou, Wen-Hui.(2013).HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,32(3),561-568.
MLA Shi, Chen-Ye,et al."HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 32.3(2013):561-568.

入库方式： OAI收割

来源：上海药物研究所

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