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Characteristic ion clusters as determinants for the identification of pyrrolizidine alkaloid N-oxides in pyrrolizidine alkaloid-containing natural products using HPLC-MS analysis

文献类型：期刊论文

作者Ruan, Jianqing2,3; Li, Na2,3; Xia, Qingsu1; Fu, Peter P.1; Peng, Shuying4; Ye, Yang3,4; Lin, Ge2,3
刊名JOURNAL OF MASS SPECTROMETRY
出版日期2012-03
卷号47期号:3页码:331-337
关键词pyrrolizidine alkaloid N-oxides pyrrolizidine alkaloid HPLC-MS analysis characteristic fragment ions fragmentation pathway
ISSN号1076-5174
DOI10.1002/jms.2969
文献子类Article
英文摘要Pyrrolizidine alkaloid (PA)containing plants are widely distributed in the world. PAs are hepatotoxic, affecting livestock and humans. PA N-oxides are often present together with PAs in plants and also exhibit hepatotoxicity but with less potency. HPLCMS is generally used to analyze PA-containing herbs, although PA references are unavailable in most cases. However, to date, without reference standards, HPLCMS methodology cannot distinguish PA N-oxides from PAs because they both produce the same characteristic ions in mass spectra. In the present study, the mass spectra of 10 PA N-oxides and the corresponding PAs were systemically investigated using HPLCMS to define the characteristic mass fragment ions specific to PAs and PA N-oxides. Mass spectra of toxic retronecine-type PA N-oxides exhibited two characteristic ion clusters at m/z 118120 and 136138. These ion clusters were produced by three unique fragmentation pathways of PA N-oxides and were not found in their corresponding PAs. Similarly, the nontoxic platynecine-type PA N-oxides also fragmented via three similar pathways to form two characteristic ion clusters at m/z 120122 and 138140. Further application of using these characteristic ion clusters allowed successful and rapid identification of PAs and PA N-oxides in two PA-containing herbal plants. Our results demonstrated, for the first time, that these characteristic ion clusters are unique determinants to discriminate PA N-oxides from PAs even without the availability of reference samples. Our findings provide a novel and specific method to differentiate PA N-oxides from PAs in PA-containing natural products, which is crucial for the assessment of their intoxication. Copyright (C) 2012 John Wiley & Sons, Ltd.
WOS关键词METABOLIC-ACTIVATION ; MASS-SPECTROMETRY ; SENECIO-SCANDENS ; DNA-ADDUCTS ; CLIVORINE ; QIANLIGUANG ; TOXICITY ; EXTRACT ; SAFETY ; RAT
资助项目Research Grant Council of Hong Kong Special Administrative Region, China (GRF)[CUHK2140690] ; National Natural Science Funds for Distinguished Young Scholar[30925043]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
WOS记录号WOS:000301575200008
出版者WILEY-BLACKWELL
源URL[http://119.78.100.183/handle/2S10ELR8/278162]  
专题中国科学院上海药物研究所
通讯作者Lin, Ge
作者单位1.Natl Ctr Toxicol Res, Jefferson, AR 72079 USA;
2.Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Joint Res Lab Promoting Globalizat Tradit Chinese, Beijing 100864, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Ruan, Jianqing,Li, Na,Xia, Qingsu,et al. Characteristic ion clusters as determinants for the identification of pyrrolizidine alkaloid N-oxides in pyrrolizidine alkaloid-containing natural products using HPLC-MS analysis[J]. JOURNAL OF MASS SPECTROMETRY,2012,47(3):331-337.
APA Ruan, Jianqing.,Li, Na.,Xia, Qingsu.,Fu, Peter P..,Peng, Shuying.,...&Lin, Ge.(2012).Characteristic ion clusters as determinants for the identification of pyrrolizidine alkaloid N-oxides in pyrrolizidine alkaloid-containing natural products using HPLC-MS analysis.JOURNAL OF MASS SPECTROMETRY,47(3),331-337.
MLA Ruan, Jianqing,et al."Characteristic ion clusters as determinants for the identification of pyrrolizidine alkaloid N-oxides in pyrrolizidine alkaloid-containing natural products using HPLC-MS analysis".JOURNAL OF MASS SPECTROMETRY 47.3(2012):331-337.

入库方式： OAI收割

来源：上海药物研究所

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