X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85 beta subunit
文献类型：期刊论文
| 作者 | Chen, Shuai2; Xiao, Yibei2,3; Ponnusamy, Rajesh2; Tan, Jinzhi2; Lei, Jian2; Hilgenfeld, Rolf1,2,4 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2011-11 |
| 卷号 | 67页码:1328-1333 |
| ISSN号 | 2053-230X |
| DOI | 10.1107/S1744309111031691 |
| 文献子类 | Article |
| 英文摘要 | Src-homology 3 (SH3) domains are involved in extensive protein-protein interactions and constitute key elements of intracellular signal transduction. Three-dimensional structures have been reported for SH3 domains of various proteins, including the 85 kDa regulatory subunit (p85) of phosphoinositide 3-kinase. However, all of the latter structures are of p85 isoform alpha and no crystal structure of the SH3 domain of the equally important isoform beta has been reported to date. In this structural communication, the recombinant production, crystallization and X-ray structure determination at 2.0 angstrom resolution of the SH3 domain of human p85 beta is described. The structure reveals a compact beta-barrel fold very similar to that of p85 alpha. However, binding studies with two classes of proline-rich ligand peptides demonstrate that the ligand-binding specificity differs slightly between the SH3 domains of human p85 beta and p85 alpha, despite their high structural similarity. |
| WOS关键词 | VIRUS NS1 PROTEIN ; PHOSPHATIDYLINOSITOL 3-KINASE ; CRYSTAL-STRUCTURE ; BINDING ; PATHWAY ; PI3K ; LIBRARIES ; PEPTIDES ; LIGANDS ; MODEL |
| 资助项目 | International Consortium on Anti-Virals (ICAV)[00000000] ; DFG Cluster of Excellence "Inflammation at Interfaces'[EXC 306] ; Chinese Academy of Sciences[2010T1S6] ; Fonds der Chemischen Industrie[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 语种 | 英语 |
| WOS记录号 | WOS:000296793300004 |
| 出版者 | INT UNION CRYSTALLOGRAPHY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278353]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hilgenfeld, Rolf |
| 作者单位 | 1.DESY, Lab Struct Biol Infect & Inflammat, D-22603 Hamburg, Germany; 2.Univ Lubeck, Ctr Struct & Cell Biol Med, Inst Biochem, D-23538 Lubeck, Germany; 3.Univ Lubeck, Grad Sch Comp Med & Life Sci, D-23538 Lubeck, Germany; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Shuai,Xiao, Yibei,Ponnusamy, Rajesh,et al. X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85 beta subunit[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2011,67:1328-1333. |
| APA | Chen, Shuai,Xiao, Yibei,Ponnusamy, Rajesh,Tan, Jinzhi,Lei, Jian,&Hilgenfeld, Rolf.(2011).X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85 beta subunit.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,67,1328-1333. |
| MLA | Chen, Shuai,et al."X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85 beta subunit".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 67(2011):1328-1333. |
入库方式： OAI收割
来源：上海药物研究所
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