Characterization of a Pectic Polysaccharide from the Leaves of Diospyros kaki and Its Modulating Activity on Lymphocyte Proliferation
文献类型：期刊论文
| 作者 | Duan, Jinyou; Dong, Qun; Ding, Kan ; Fang, Jinian
|
| 刊名 | BIOPOLYMERS
 |
| 出版日期 | 2010-07 |
| 卷号 | 93期号:7页码:649-656 |
| 关键词 | pectin rhamnogalacturonan immunological activity Diospyros kaki |
| ISSN号 | 0006-3525 |
| DOI | 10.1002/bip.21430 |
| 文献子类 | Article |
| 英文摘要 | Pectin is a group of carbohydrate polymers constructing the primary cell walls and the middle lamella of terrestrial plants. Herein, we demonstrated the structure and immunomodulatory activity of the major Pectic polysaccharide DL-3B(2) isolated from the leaves of Diospyros kaki. Based on composition analysis, methylation analysis, two-step acid hydrolysis, lithium-mediated selective degradation, (13)C NMR spectroscopy, and electrospray ionization mass spectrometry, DL-3B(2) was found to contain an alpha-1, 4-linked galacturonic acid (GalA) backbone with some insertions of alpha-1, 2-linked rhamnose residues. The arabinan- and arabinogalactan-side chains were attached to O-4 of the rhamnose residues, whereas the linear arabinoxylan was probably linked to O-3 of the GalA residues. Immunological tests in vitro showed that DL-3B(2) could help stimulate lipopolysaccharide-induced B lymphocyte proliferation, but not ConA-induced T lymphocyte proliferation, and that the arabinose residues play a role in maintaining this immunological activity (C) 2010 Wiley Periodicals, Inc. Biopolymers 93: 649-656, 2010. |
| WOS关键词 | STRUCTURAL FEATURES ; ACID RESIDUES ; IN-VITRO ; CELLS ; DEGRADATION ; HOMOGALACTURONAN ; ARABINOGALACTAN ; METHYLATION ; SUBSTANCES ; COMPONENTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000277983900006 |
| 出版者 | JOHN WILEY & SONS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278848]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Duan, Jinyou |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Jinyou,Dong, Qun,Ding, Kan,et al. Characterization of a Pectic Polysaccharide from the Leaves of Diospyros kaki and Its Modulating Activity on Lymphocyte Proliferation[J]. BIOPOLYMERS,2010,93(7):649-656. |
| APA | Duan, Jinyou,Dong, Qun,Ding, Kan,&Fang, Jinian.(2010).Characterization of a Pectic Polysaccharide from the Leaves of Diospyros kaki and Its Modulating Activity on Lymphocyte Proliferation.BIOPOLYMERS,93(7),649-656. |
| MLA | Duan, Jinyou,et al."Characterization of a Pectic Polysaccharide from the Leaves of Diospyros kaki and Its Modulating Activity on Lymphocyte Proliferation".BIOPOLYMERS 93.7(2010):649-656. |
入库方式： OAI收割
来源：上海药物研究所
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