Investigation of the effects of 24 bio-matrices on the LC-MS/MS analysis of morinidazole
文献类型：期刊论文
| 作者 | Lv, Wenxu |
| 刊名 | TALANTA
 |
| 出版日期 | 2010-01-15 |
| 卷号 | 80期号:3页码:1406-1412 |
| 关键词 | Matrix effects Bio-matrices Pulse gradient with ammonium formate effects Pharmacokinetic study LC-ESI-MS/MS Protein precipitation |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2009.09.043 |
| 文献子类 | Article |
| 英文摘要 | This study compares and evaluates the effect of various matrices on liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) analysis. Permanent post-column infusion (PCI) was used to quantify matrix effects. In this way, the suppressed or enhanced signal of the target material resulting from different co-eluting matrix components could be assessed. Twenty-four biological samples from in vivo and in vitro experiments were selected for this study. In addition, 7 sample components were further analyzed after sample preparation by protein precipitation. Multiple regression analysis was used to investigate the collinear relationship between matrix effects and co-eluted components at different time intervals. We found that salt was the dominant factor which impacted changes in signal detection. In order to eliminate it, we used ammonium formate as a modifier of the mobile phase which resulted in charge-state redistribution profiles so that a homogeneous matrix formed. By employing pulse gradient chromatography in the presence of 5 mM ammonium formate, favorable improvements of enhanced signal intensity and reduced matrix effects were obtained. These experiments also indicated the feasibility of using analogue IS during bio-analysis which contributed to an overall faster assay that would be suitable for drug discovery and development purposes. (C) 2009 Elsevier B.V. All rights reserved |
| WOS关键词 | LABELED INTERNAL STANDARD ; MASS-SPECTROMETRIC METHOD ; HUMAN PLASMA ; QUANTITATIVE BIOANALYSIS ; ELECTROSPRAY-IONIZATION ; LC/MS/MS ANALYSES ; DRUG DISCOVERY ; LIQUID ; PERFORMANCE ; QUANTIFICATION |
| 资助项目 | Chinese Ministry of Science and Technology[2004CB518902] ; Chinese Ministry of Science and Technology[2005CB523403] ; Science & Technology Commission of Shanghai Municipality[04DZ19215] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000273929900055 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278996]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Lv, Wenxu |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Materia Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Wenxu. Investigation of the effects of 24 bio-matrices on the LC-MS/MS analysis of morinidazole[J]. TALANTA,2010,80(3):1406-1412. |
| APA | Lv, Wenxu.(2010).Investigation of the effects of 24 bio-matrices on the LC-MS/MS analysis of morinidazole.TALANTA,80(3),1406-1412. |
| MLA | Lv, Wenxu."Investigation of the effects of 24 bio-matrices on the LC-MS/MS analysis of morinidazole".TALANTA 80.3(2010):1406-1412. |
入库方式： OAI收割
来源：上海药物研究所
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