The negatively charged amino acids in the lumenal loop influence the pigment binding and conformation of the major light-harvesting chlorophyll a/b complex of photosystem II
文献类型：期刊论文
| 作者 | Yang, Chunhong2,3; Lambrev, Petar4; Chen, Zhi1,5; Javorfi, Tamas; Kiss, Anett Z.4; Paulsen, Harald3; Garab, Gyoezoe4 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
 |
| 出版日期 | 2008-11 |
| 卷号 | 1777期号:11页码:1463-1470 |
| ISSN号 | 0005-2728 |
| 关键词 | Major light-harvesting a/b complex of photosystem II Spectroscopy Mutagenesis Low pH |
| DOI | 10.1016/j.bbabio.2008.08.009 |
| 文献子类 | Article |
| 英文摘要 | The major chlorophyll (Chl) a/b complexes of photosystem II (LHCIIb), in addition to their primary light-harvesting function, play key roles in the organization of the granal ultrastructure of the thylakoid membranes and in various regulatory processes. These functions depend on the structural stability and flexibility of the complexes. The lumenal side of LHCIIb is exposed to broadly variable pH environments, due to the build-up and decay of the pH gradient during photosynthesis. Therefore, the negatively charged amino acids in the lumenal loop might be of paramount importance for adjusting the structure and functions of LHCIIb. In order to clarify the structural roles of these residues, we investigated the pigment stoichiometries, absorption, linear and circular dichroism spectra of the reconstituted LHCIIb complexes, in which the negatively charged amino acids in the lumenal loop were exchanged to neutral ones (E94G, E107V and DIM). The mutations influenced the pigment binding and the molecular architecture of the complexes. Exchanging E94 to G destabilized the 3(10) helix in the lumenal loop structure and led to an acquired pH sensitivity of the LHCIIb structure. We conclude that these amino acids are important not only for pigment binding in the complexes, but also in stabilizing the conformation of LHCIIb at different pHs. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | MOLECULAR-DYNAMICS ; HIGHER-PLANTS ; THYLAKOID STRUCTURE ; NEOXANTHIN BINDING ; THERMAL-STABILITY ; PROTEIN COMPLEX ; LHCII ; ANTENNA ; STOICHIOMETRY ; MACRODOMAINS |
| 资助项目 | Marie Curie[MCRTN-CF-2003-505069] ; Hungarian Fund for Basic Research[OTKA K63252] ; Deutsche Forschungsgemeinschaft[00000000] ; National Natural Science Foundation of China[30470149] ; Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-137] ; EMBO Shortterm Fellowships[ASTF 190-04] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000261080200009 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279441]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Chunhong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Ctr Drug Discovery & Design,State Key Lab Drug Re, Beijing 100864, Peoples R China; 2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China; 3.Johannes Gutenberg Univ Mainz, Inst Allgemeine Bot, D-55099 Mainz, Germany; 4.Hungarian Acad Sci, Inst Plant Biol, H-6701 Szeged, Hungary; 5.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Chunhong,Lambrev, Petar,Chen, Zhi,et al. The negatively charged amino acids in the lumenal loop influence the pigment binding and conformation of the major light-harvesting chlorophyll a/b complex of photosystem II[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2008,1777(11):1463-1470. |
| APA | Yang, Chunhong.,Lambrev, Petar.,Chen, Zhi.,Javorfi, Tamas.,Kiss, Anett Z..,...&Garab, Gyoezoe.(2008).The negatively charged amino acids in the lumenal loop influence the pigment binding and conformation of the major light-harvesting chlorophyll a/b complex of photosystem II.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1777(11),1463-1470. |
| MLA | Yang, Chunhong,et al."The negatively charged amino acids in the lumenal loop influence the pigment binding and conformation of the major light-harvesting chlorophyll a/b complex of photosystem II".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1777.11(2008):1463-1470. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。