TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury
文献类型：期刊论文
| 作者 | Liu, Aifen2; Wu, Jing3; Yang, Cheng4,5; Wu, Yuanyuan6; Zhang, Yufang2; Zhao, Fengbo2; Wang, Hui3; Yuan, Li3; Song, Lirui7,8; Zhu, Tongyu4,5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-04-03 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-22852-2 |
| 文献子类 | Article |
| 英文摘要 | Transient receptor potential melastatin 7 (TRPM7) is a membrane ion channel and kinase. TRPM7 was abundantly expressed in the kidney, and up-regulated by ischemia reperfusion (IR) injury. Our previous studies showed that cyclic helix B peptide (CHBP) improved renal IR-related injury, but its underlying mechanism is not well defined. IR-related injury was established in renal tubular epithelial cells (TCMK-1 and HK-2) via 12 to 24-h hypoxia (H) followed by 2-24 h reoxygenation (R), and in mouse kidneys subjected to 30-min ischemia and 12-h to 7-day reperfusion. TRPM7-like current in TCMK-1 cells, TRPM7 mRNA and protein in the in vitro and in vivo models were increased, but reversed by CHBP. TRPM7 was also positively associated with LDH, HMGB1, caspase-3, Bax/Bcl-2, inflammation, apoptosis, tubulointerstitial damage and renal function respectively. Furthermore, silencing TRPM7 improved injury parameters, renal histology and function in the both models. Specific TRPM7 agonist, bradykinin, exaggerated HR induced injury in TCMK-1 cells, and partially blocked the renoprotection of CHBP as well. In conclusion, TRPM7 is involved not only in IR-related injury, but also CHBP-induced renoprotection, which are through its ion channel and subsequent affects inflammation and apoptosis. Therefore, TRPM7 could be a potential biomarker for IR-induced acute kidney injury. |
| WOS关键词 | RENAL ISCHEMIA/REPERFUSION INJURY ; POTENTIAL MELASTATIN 7 ; ACUTE KIDNEY INJURY ; HELIX B PEPTIDE ; CELL-DEATH ; NEURONAL DEATH ; RECEPTOR 4 ; RAT MODEL ; APOPTOSIS ; ERYTHROPOIETIN |
| 资助项目 | National Natural Science Foundation of China[81300564] ; National Natural Science Foundation of China[81570677] ; National Natural Science Foundation of China[81170689] ; National Natural Science Foundation of China[81400752] ; National Natural Science Foundation of China[81402189] ; National Natural Science Foundation of China[81270833] ; National Natural Science Foundation of China[81570674] ; Science and Technology Projects of Nantong[MS12015117] ; Natural Science Foundation of Jiangsu Province[BK20140437] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000428999200046 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279812]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Bin |
| 作者单位 | 1.Univ Leicester, Leicester Gen Hosp, Univ Hosp Leicester, Dept Infect Immun & Inflammat, Leicester LE1 9HN, Leics, England 2.Nantong Univ, Med Coll, Basic Med Res Ctr, Renal Grp, Nantong 226001, Jiangsu, Peoples R China; 3.Nantong Univ, Affiliated Hosp, Dept Nephrol, Nantong 226001, Jiangsu, Peoples R China; 4.Fudan Univ, Zhongshan Hosp, Dept Urol, Shanghai 200032, Peoples R China; 5.Shanghai Key Lab Organ Transplantat, Shanghai 200032, Peoples R China; 6.Nantong Univ, Med Coll, Dept Pathol, Nantong 226001, Jiangsu, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Aifen,Wu, Jing,Yang, Cheng,et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Liu, Aifen.,Wu, Jing.,Yang, Cheng.,Wu, Yuanyuan.,Zhang, Yufang.,...&Yang, Bin.(2018).TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury.SCIENTIFIC REPORTS,8. |
| MLA | Liu, Aifen,et al."TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：上海药物研究所
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