Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa
文献类型：期刊论文
| 作者 | Liu, Hui2; Duan, Wen-Di1,3; Ribeiro de Souza, Fayene Zeferino4; Liu, Lan2; Chen, Bi-Shuang2 |
| 刊名 | CATALYSTS
 |
| 出版日期 | 2018-04 |
| 卷号 | 8期号:4 |
| 关键词 | biocatalyst Rhodotorula mucilaginosa immobilization asymmetric reduction stability |
| ISSN号 | 2073-4344 |
| DOI | 10.3390/catal8040165 |
| 文献子类 | Article |
| 英文摘要 | In our previous study, Rhodotorula mucilaginosa (R. mucilaginosa) was selected via high throughput screening as a very active and selective whole-cell biocatalyst for the asymmetric reduction of ketones. In this study, the reduction of ketones to the desired chiral alcohols by immobilized cells of this strain was investigated. Characterization with Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that whole R. mucilaginosa cells were successfully immobilized on support matrices composed of agar, calcium alginate, PVA-alginate and chitosan. The immobilized cells were applied to the enantioselective reduction of fourteen different aromatic ketones. Good to excellent results were achieved with R. mucilaginosa cells immobilized on agar and calcium alginate. The immobilized cells on the selected support matrix composed of agar exhibited a significant increase in pH tolerance at pH 3.5-9 and demonstrated highly improved thermal stability compared to free cells. The cells immobilized on agar retained 90% activity after 60 days storage at 4 degrees C and retained almost 100% activity after 6 reuse cycles. In addition, the immobilization procedures are very simple and cause minimal pollution. These results suggest that the application of immobilized R. mucilaginosa can be practical on an industrial scale to produce chiral alcohols. |
| WOS关键词 | MARINE-DERIVED FUNGI ; CHITOSAN ; ACID ; ALCOHOLS ; CELLS ; BIOREDUCTION ; CHITIN ; BEADS |
| 资助项目 | Natural Science Foundation of Guangdong Province[2017A030310232] ; National Natural Science Foundation of China[41706148] ; Basic Research Program of Sun Yat-Sen University[17lgpy58] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09735010] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000435186400042 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279816]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Bi-Shuang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Sun Yat Sen Univ, Sch Marine Sci, Guangzhou 510275, Guangdong, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 210203, Peoples R China; 4.UNESP, Fac Ciencias, Dept Quim, BR-17033360 Bauru, Brazil |
| 推荐引用方式 GB/T 7714 | Liu, Hui,Duan, Wen-Di,Ribeiro de Souza, Fayene Zeferino,et al. Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa[J]. CATALYSTS,2018,8(4). |
| APA | Liu, Hui,Duan, Wen-Di,Ribeiro de Souza, Fayene Zeferino,Liu, Lan,&Chen, Bi-Shuang.(2018).Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa.CATALYSTS,8(4). |
| MLA | Liu, Hui,et al."Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa".CATALYSTS 8.4(2018). |
入库方式： OAI收割
来源：上海药物研究所
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