Screening of neuraminidase inhibitory activities of some medicinal plants traditionally used in Lingnan Chinese medicines
文献类型：期刊论文
| 作者 | Liu, Jiawei2; Zu, Mian1,3; Chen, Kaotan2; Gao, Li1,3; Min, Huan2; Zhuo, Weiling1,3; Chen, Weiwen2; Liu, Ailin1,3 |
| 刊名 | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
 |
| 出版日期 | 2018-03-20 |
| 卷号 | 18 |
| ISSN号 | 1472-6882 |
| 关键词 | A (H1N1) influenza virus Neuraminidase inhibitor Anti-influenza agents Medicinal plant Lingnan Chinese medicines |
| DOI | 10.1186/s12906-018-2173-1 |
| 文献子类 | Article |
| 英文摘要 | Background: Neuraminidase (NA) is one of the key surface protein of the influenza virus, and has been established as a primary drug target for anti-influenza therapies. This study aimed to screen bioactive herbal extracts from some medicinal plants traditionally used in Lingnan Chinese Medicines by NA activity high-throughput screening assay. Methods: One hundred ninety herbal extracts from 95 medicinal plants collected in Guangzhou were screened for their potential inhibitory activities against A (H1N1) influenza neuraminidase, and the most active extracts were further evaluated for their anti-influenza virus activities using virus-induced cytopathic effect (CPE). Results: Among the tested 190 herbal extracts, 14 extracts inhibited significantly NA activity (IC50 < 40 mu g/mL), and the extracts 1-5, which were obtained from Amomurn villosum Lour, Melaphis chinensis (Bell) Baker, Sanguisorba officinalis and Flos Caryophylli, showed potent inhibitory activity against NA with IC50 values ranging from 4.1 to 9.6 mu g/mL. Moreover, the most bioactive extracts 1-5 were found to protect MDCK cells from A (H1N1) influenza virus infection with very low cytotoxicity to the host cells (EC50 values ranged from 1.8 to 14.1 mu g/mL, CC50 values ranged from 97.0 to 779.2 mu g/mL, SI values ranged from 14 to 438). In addition, quantitative RT-PCR analysis showed that the extracts 1-5 inhibited viral RNA synthesis in a dose-dependent manner. Conclusion: We performed in vitro screening of anti-neuraminidase activities of herbal extracts from medicinal plants used in Lingnan Chinese Medicines, and the results indicate that some bioactive extracts are worth further studies to identify the bioactive components responsible for anti-influenza virus activities, to elucidate their modes of action and finally determine their clinical potentials. |
| WOS关键词 | INFLUENZA-VIRUS NEURAMINIDASE ; SANGUISORBA-OFFICINALIS L. ; ANTIVIRAL ACTIVITIES ; ETHANOL EXTRACT ; OSELTAMIVIR ; FLAVONOIDS ; EMERGENCE ; ZANAMIVIR ; VACCINES ; TARGET |
| 资助项目 | China National Natural Science Foundation[81373432] ; Guangzhou Science and Technology Program[2014 J4100118] ; National Great Science and Technology Major Projects[2012ZX09301002-2013HXW-11] ; Beijing Natural Science Foundation Grant[7152103] |
| WOS研究方向 | Integrative & Complementary Medicine |
| 语种 | 英语 |
| 出版者 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000428224900007 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279845]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Jiawei; Liu, Ailin |
| 作者单位 | 1.Chinese Acad Sci, Beijing Key Lab Drug Target Res & Drug Screening, Inst Mat Med, 1 Xian Nong Tan St, Beijing 100050, Peoples R China; 2.Guangzhou Univ Chinese Med, Res Ctr Med Plants Resource Sci & Engn, Key Lab Chinese Med Resource Lingnan, Minist Educ,Higher Educ Mega Ctr, 232 Waihuandong Rd, Guangzhou 510006, Guangdong, Peoples R China; 3.Peking Union Med Coll, 1 Xian Nong Tan St, Beijing 100050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jiawei,Zu, Mian,Chen, Kaotan,et al. Screening of neuraminidase inhibitory activities of some medicinal plants traditionally used in Lingnan Chinese medicines[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2018,18. |
| APA | Liu, Jiawei.,Zu, Mian.,Chen, Kaotan.,Gao, Li.,Min, Huan.,...&Liu, Ailin.(2018).Screening of neuraminidase inhibitory activities of some medicinal plants traditionally used in Lingnan Chinese medicines.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,18. |
| MLA | Liu, Jiawei,et al."Screening of neuraminidase inhibitory activities of some medicinal plants traditionally used in Lingnan Chinese medicines".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 18(2018). |
入库方式： OAI收割
来源：上海药物研究所
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