An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
文献类型：期刊论文
| 作者 | Lim, Nastasia K-H2,3; Moestrup, Visse4,5; Zhang, Xiao2; Wang, Wen-An6; Moller, Arne1,4,5,7; Huang, Fu-De2,5 |
| 刊名 | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
 |
| 出版日期 | 2018-03 |
| 期号 | 133 |
| 关键词 | Neuroscience Issue 133 Cerebrospinal fluid mouse neuroscience brain central nervous system tissue extraction |
| ISSN号 | 1940-087X |
| DOI | 10.3791/56774 |
| 文献子类 | Article |
| 英文摘要 | The cerebrospinal fluid (CSF) is a valuable body fluid for analysis in neuroscience research. It is one of the fluids in closest contact with the central nervous system and thus, can be used to analyze the diseased state of the brain or spinal cord without directly accessing these tissues. However, in mice it is difficult to obtain from the cisterna magna due to its closeness to blood vessels, which often contaminate samples. The area for CSF collection in mice is also difficult to dissect to and often only small samples are obtained (maximum of 5-7 mu L or less). This protocol describes in detail a technique that improves on current methods of collection to minimize contamination from blood and allow for the abundant collection of CSF (on average 10-15 mu L can be collected). This technique can be used with other dissection methods for tissue collection from mice, as it does not impact any tissues during CSF extraction. Thus, the brain and spinal cord are not affected with this technique and remain intact. With greater CSF sample collection and purity, more analyses can be used with this fluid to further aid neuroscience research and better understand diseases affecting the brain and spinal cord. |
| WOS关键词 | BIOMARKERS ; DIAGNOSIS ; DISEASE |
| 资助项目 | National Natural Science Foundation of China[81650110527] ; National Natural Science Foundation of China[81371400] ; National Key Basic Research Program of China[2013CB530900] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000443329500126 |
| 出版者 | JOURNAL OF VISUALIZED EXPERIMENTS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279859]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Lim, Nastasia K-H; Huang, Fu-De |
| 作者单位 | 1.Aarhus Univ Hosp, Dept Nucl Med, Aarhus, Denmark; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Adv Res Inst, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Mat Med, Beijing, Peoples R China; 4.Aarhus Univ, Dept Clin Med, Ctr Functionally Integrat Neurosci, Aarhus, Denmark; 5.Sinodanish Ctr Educ & Res SDC, Beijing, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Neurol,Chongming Branch, Shanghai, Peoples R China; 7.Aarhus Univ Hosp, PET Ctr, Aarhus, Denmark |
| 推荐引用方式 GB/T 7714 | Lim, Nastasia K-H,Moestrup, Visse,Zhang, Xiao,et al. An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2018(133). |
| APA | Lim, Nastasia K-H,Moestrup, Visse,Zhang, Xiao,Wang, Wen-An,Moller, Arne,&Huang, Fu-De.(2018).An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(133). |
| MLA | Lim, Nastasia K-H,et al."An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .133(2018). |
入库方式： OAI收割
来源：上海药物研究所
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