Characterizing the interactions of two lipid modifications with lipid rafts: farnesyl anchors vs. palmitoyl anchors
文献类型：期刊论文
| 作者 | Zhang, Tao2; Luo, Qichao1,3; Yang, Linlin4; Jiang, Hualiang1,2,3 ; Yang, Huaiyu3
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| 刊名 | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
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| 出版日期 | 2018-01 |
| 卷号 | 47期号:1页码:19-30 |
| 关键词 | Lipid modifications Lipid rafts Non-raft-like membranes Molecular dynamics simulations Interactions |
| ISSN号 | 0175-7571 |
| DOI | 10.1007/s00249-017-1217-7 |
| 文献子类 | Article |
| 英文摘要 | Farnesyl (Far) and palmitoyl (Pal) anchors play important roles in the traffic of many lipidated proteins. Herein, we show the distinctive interactions and influences of the two lipid modifications on lipid rafts (LRs) and non-raft-like membranes using molecular dynamics simulations. Palmitoyl anchors behave in a more ordered fashion, pack tighter with the lipids of LRs and diffuse at a slower rate than farnesyl anchors in LRs. When interacting with non-raft-like membranes these two types of anchors become less ordered, pack more loosely with lipids, and diffuse at a higher rate. By calculating both the number of contacts per chain and the number of contact atoms per carbon of the two anchors with the lipid components, we found that the palmitoyl chains preferred to associate with the saturated chains of lipids and cholesterol molecules in LRs, while farnesyl chains favored association with saturated chains and unsaturated chains. For non-raft-like membranes, these two lipid anchors had roughly the same preference for the three types of contact lipid chains. Additionally, palmitoyl anchors caused cholesterol to orient more perpendicular to the membrane surface, surrounding lipids to become more ordered, and lipid lateral fluidity to reduce significantly, compared to farnesyl anchors in LRs. By contrast, the POPE and DSPC became much less ordered, cholesterol became more tilted, and lipids became more fluid, when the two types lipid anchors were inserted in non-raft-like membranes. These findings are useful for understanding the traffic mechanisms of lipidated proteins with farnesyl and palmitoyl modifications in cell membranes. |
| WOS关键词 | PROTEIN PRENYLATION ; MEMBRANE RAFTS ; BIOLOGICAL IMPLICATION ; SIGNAL-TRANSDUCTION ; MOLECULAR-DYNAMICS ; BILAYER-MEMBRANES ; FATTY ACYLATION ; N-RAS ; CHOLESTEROL ; DOMAINS |
| 资助项目 | Ministry of Science and Technology of China[2012CB518005] ; Ministry of Science and Technology of China[2015CB910304] ; National Natural Science Foundation of China[21422208] ; E-Institutes of Shanghai Municipal Education Commission[E09013] ; Institutes for Drug Discovery and Development, Chinese Academy of Sciences[00000000] |
| WOS研究方向 | Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000419474600003 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272346]  |
| 专题 | 药物发现与设计中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Jiang, Hualiang; Yang, Huaiyu |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.East China Univ Sci & Technol, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 4.Zhengzhou Univ, Sch Basic Med Sci, Dept Pharmacol, 100 Sci Ave, Zhengzhou 450001, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Tao,Luo, Qichao,Yang, Linlin,et al. Characterizing the interactions of two lipid modifications with lipid rafts: farnesyl anchors vs. palmitoyl anchors[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2018,47(1):19-30. |
| APA | Zhang, Tao,Luo, Qichao,Yang, Linlin,Jiang, Hualiang,&Yang, Huaiyu.(2018).Characterizing the interactions of two lipid modifications with lipid rafts: farnesyl anchors vs. palmitoyl anchors.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,47(1),19-30. |
| MLA | Zhang, Tao,et al."Characterizing the interactions of two lipid modifications with lipid rafts: farnesyl anchors vs. palmitoyl anchors".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 47.1(2018):19-30. |
入库方式： OAI收割
来源：上海药物研究所
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