An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance
文献类型：期刊论文
| 作者 | Jiang, Yuejing2,4; Cheng, Juan3; Yang, Chengyu1; Hu, Yongzhou3; Li, Jia4 ; Han, Yifeng1; Zang, Yi4; Li, Xin3
|
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 8期号:12页码:8012-8018 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c7sc03338a |
| 文献子类 | Article |
| 英文摘要 | Unveiling the detailed roles of glutathione (GSH) in chemoresistance necessitates a reliable assay for its detection in intact live specimens. Herein, by taking advantage of the susceptibility of electron-poor C-sp2-S-sufinyl bond to GSH nucleophilic attack, we developed a naphthalimide-sulfoxide based fluorogenic probe (Na-8) applicable for tracking endogenous GSH fluctuation in live cells. Na-8 features a high degree of sensitivity towards GSH as demonstrated by its utmost 2200-fold fluorogenic response. As a proof of concept, Na-8 has been applied to image GSH in liver cancer HepG2 cells with the normal L02 cell counterparts serving as a control, and elevated GSH level was observed in HepG2 in contrast to L02. Further experiments showed that this elevated GSH level was involved in doxorubicin-resistance but not in cisplatin-resistance. Noteworthy, monitoring GSH change in HepG2 and L02 cells in response to doxorubicin treatment revealed that while normal cells showed a burst of GSH in adaption to doxorubicin treatment, no significant change was detected in HepG2 cells, suggesting that HepG2 cells have been preconditioned by their intrinsic oxidative stress which confers drug-resistance. Given the observed sensitivity and spatiotemporal resolution, Na-8 should hold promise for future study on the detailed roles of GSH in drug-resistance. |
| WOS关键词 | REVERSIBLE FLUORESCENT-PROBES ; DRUG-RESISTANCE ; BUTHIONINE SULFOXIMINE ; CELLULAR GLUTATHIONE ; CANCER ; CELLS ; CISPLATIN ; MELPHALAN ; ACCEPTOR ; PROTEIN |
| 资助项目 | National Natural Science Foundations of China[21642007] ; National Natural Science Foundations of China[81673489] ; National Natural Science Foundations of China[21778048] ; Zhejiang Provincial Natural Science Foundation of China[LY15H300003] ; Zhejiang Provincial Natural Science Foundation of China[LR18H300001] ; Science and Technology Commission of Shanghai Municipality[16430724100] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000415877000016 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272382]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zang, Yi; Li, Xin |
| 作者单位 | 1.Zhejiang Sci Tech Univ, Dept Chem, Hangzhou 310018, Zhejiang, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Jiang, Yuejing,Cheng, Juan,Yang, Chengyu,et al. An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance[J]. CHEMICAL SCIENCE,2017,8(12):8012-8018. |
| APA | Jiang, Yuejing.,Cheng, Juan.,Yang, Chengyu.,Hu, Yongzhou.,Li, Jia.,...&Li, Xin.(2017).An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance.CHEMICAL SCIENCE,8(12),8012-8018. |
| MLA | Jiang, Yuejing,et al."An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance".CHEMICAL SCIENCE 8.12(2017):8012-8018. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。