Adduct ion-targeted qualitative and quantitative analysis of polyoxypregnanes by ultra-high pressure liquid chromatography coupled with triple quadrupole mass spectrometry
文献类型：期刊论文
| 作者 | Wu, X.2,3,5; Zhu, Lin3,5; Ma, Jiang3,5; Ye, Yang1,4,5 ; Lin, Ge3,5
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2017-10-25 |
| 卷号 | 145页码:127-136 |
| 关键词 | Adduct ion formation Marsdenia tenacissima Polyoxypregnane Triple quadrupole mass spectrometry |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2017.06.038 |
| 文献子类 | Article |
| 英文摘要 | Polyoxypregnane and its glycosides (POPs) are frequently present in plants of Asclepiadaceae family, and have a variety of biological activities. There is a great need to comprehensively profile these phytochemicals and to quantify them for monitoring their contents in the herbs and the biological samples. However, POPs undergo extensive adduct ion formation in ESI-MS, which has posed a challenge for qualitative and quantitative analysis of POPs. In the present study, we took the advantage of such extensive adduct ion formation to investigate the suitability of adduct ion -targeted analysis of POPs. For the qualitative analysis, we firstly demonstrated that the sodium and ammonium adduct ion -targeted product ion scans (PIS) provided adequate MS/MS fragmentations for structural characterization of POPs. Aided with precursor ion (PI) scans, which showed high selectivity and sensitivity and improved peak assignment confidence in conjunction with full scan (FS), the informative adduct ion -targeted PIS enabled rapid POPs profiling. For the quantification, we used formic acid rather than ammonium acetate as an additive in the mobile phase to avoid simultaneous formation of sodium and ammonium adduct ions, and greatly improved reproducibility of MS response of POPs. By monitoring the solely formed sodium adduct ions [M+Na](+), a method for simultaneous quantification of 25 POPs in the dynamic multiple reaction monitoring mode was then developed and validated. Finally, the aforementioned methods were applied to qualitative and quantitative analysis of POPs in the extract of a traditional Chinses medicinal herb, Marsdenia tenacissima (Roxb.) Wight et Am., and in the plasma obtained from the rats treated with this herb. The results demonstrated that adduct ion formation could be optimized for the qualitative and quantitative analysis of POPs, and our developed PI/FS-PIS scanning and sole [M+Na](+) ion monitoring significantly improved the analysis of POPs in both herbal and biological samples. This study also provides implications for the analysis of other compounds which undergo extensive adduct ion formation in ESI-MS. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS关键词 | NEUTRAL LOSS SCAN ; MARSDENIA-TENACISSIMA ; PREGNANE GLYCOSIDES ; STRUCTURAL-CHARACTERIZATION ; STEROIDAL GLYCOSIDES ; DREGEA-VOLUBILIS ; IDENTIFICATION ; STEMS ; QUANTIFICATION ; EXTRACT |
| 资助项目 | Direct Grant[2041448] ; CUHK School of Biomedical Science-Seed Fund for Joint Establishments (SBS-SIMM)[00000000] ; One-off Funding for joint Lab/Research Collaboration[3132968] ; Chinese University of Hong Kong, Hong Kong[00000000] ; Health and Medical Research Fund[08090481] ; Food and Health Bureau, Hong Kong[00000000] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000410872200017 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272442]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Lin, Ge |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 2.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Lab Mol Pharmacol, Luzhou, Sichuan, Peoples R China 3.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai, Peoples R China; 5.Chinese Acad Sci, Joint Res Lab Promoting Globalizat Tradit Chinese, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wu, X.,Zhu, Lin,Ma, Jiang,et al. Adduct ion-targeted qualitative and quantitative analysis of polyoxypregnanes by ultra-high pressure liquid chromatography coupled with triple quadrupole mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,145:127-136. |
| APA | Wu, X.,Zhu, Lin,Ma, Jiang,Ye, Yang,&Lin, Ge.(2017).Adduct ion-targeted qualitative and quantitative analysis of polyoxypregnanes by ultra-high pressure liquid chromatography coupled with triple quadrupole mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,145,127-136. |
| MLA | Wu, X.,et al."Adduct ion-targeted qualitative and quantitative analysis of polyoxypregnanes by ultra-high pressure liquid chromatography coupled with triple quadrupole mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 145(2017):127-136. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。