GPCR Activation and Endocytosis Induced by a 2D Material Agonist
文献类型：期刊论文
| 作者 | Dou, Wei-Tao1,2; Kong, Ya3,4; He, Xiao-Peng1,2; Chen, Guo-Rong1,2; Zang, Yi3,4 ; Li, Jia3,4; Tian, He1,2
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2017-05-03 |
| 卷号 | 9期号:17页码:14709-14715 |
| 关键词 | 2D material G protein-coupled receptor ligand peptide fluorescence imaging photodynamic therapy endocytosis |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.7b02754 |
| 文献子类 | Article |
| 英文摘要 | Agonist-induced activation and endocytosis of G protein-coupled receptors (GPCRs) are crucial for a number of physiological and pathological processes. However, tools that are available for probing GPCR endocytosis have been insufficient. Here, we developed,a two-dimensional (212) material agonist by supramolecular self-assembly between an endogenous agonist of K-opioid receptor (KOR) and 21) molybdenum disulfide. The 2D material agonist has proven to, be amenable for eliciting GPCR activation and endocytosis in cells stably expressing KOR rather than in those without KOR expression. Using super-resolution Microscopy, we also Show that the 2D material agonist colocalizes well with GFP-fused KOR intracellularly. Further, the endocytosed 2D,material agonist can selectively produce reactive oxygen species in cells that overly express KOR, as controlled by light irradiation. |
| WOS关键词 | PROTEIN-COUPLED RECEPTORS ; TRANSITION-METAL DICHALCOGENIDES ; KAPPA-OPIOID RECEPTOR ; PEGYLATED MOS2 NANOSHEETS ; BOTTOM-UP SYNTHESIS ; DRUG DISCOVERY ; CANCER-THERAPY ; BIOMEDICAL APPLICATIONS ; PHOTODYNAMIC THERAPY ; DYNORPHIN PEPTIDES |
| 资助项目 | 973 project[2013CB733700] ; Natural Science Foundation of China[21572058] ; Natural Science Foundation of China[21576088] ; Natural Science Foundation of China[81673489] ; Shanghai Science and Technology Development Funds[16430724100] ; Fundamental Research Funds for Central Universities[222201717003] ; Shanghai Rising-Star Program[16QA1401400] |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000400802700018 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272671]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物安全性评价中心 |
| 通讯作者 | He, Xiao-Peng; Zang, Yi; Li, Jia |
| 作者单位 | 1.East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China; 2.East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China; 4.UCAS, 19A,Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dou, Wei-Tao,Kong, Ya,He, Xiao-Peng,et al. GPCR Activation and Endocytosis Induced by a 2D Material Agonist[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(17):14709-14715. |
| APA | Dou, Wei-Tao.,Kong, Ya.,He, Xiao-Peng.,Chen, Guo-Rong.,Zang, Yi.,...&Tian, He.(2017).GPCR Activation and Endocytosis Induced by a 2D Material Agonist.ACS APPLIED MATERIALS & INTERFACES,9(17),14709-14715. |
| MLA | Dou, Wei-Tao,et al."GPCR Activation and Endocytosis Induced by a 2D Material Agonist".ACS APPLIED MATERIALS & INTERFACES 9.17(2017):14709-14715. |
入库方式： OAI收割
来源：上海药物研究所
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