Ginsenoside Rb2 Alleviates Hepatic Lipid Accumulation by Restoring Autophagy via Induction of Sirt1 and Activation of AMPK
文献类型：期刊论文
| 作者 | Huang, Qi1,2; Wang, Ting1; Yang, Liu1,2; Wang, He-Yao1
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2017-05 |
| 卷号 | 18期号:5 |
| 关键词 | ginsenoside Rb2 NAFLD type 2 diabetes hepatosteatosis autophagy AMPK sirt1 |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms18051063 |
| 文献子类 | Article |
| 英文摘要 | Although Panax ginseng is a famous traditional Chinese medicine and has been widely used to treat a variety of metabolic diseases including hyperglycemia, hyperlipidemia, and hepatosteatosis, the effective mediators and molecular mechanisms remain largely unknown. In this study we found that ginsenoside Rb2, one of the major ginsenosides in Panax ginseng, was able to prevent hepatic lipid accumulation through autophagy induction both in vivo and in vitro. Treatment of male db/db mice with Rb2 significantly improved glucose tolerance, decreased hepatic lipid accumulation, and restored hepatic autophagy. In vitro, Rb2 (50 mu mol/L) obviously increased autophagic flux in HepG2 cells and primary mouse hepatocytes, and consequently reduced the lipid accumulation induced by oleic acid in combination with high glucose. Western blotting analysis showed that Rb2 partly reversed the high fatty acid in combination with high glucose (OA)-induced repression of autophagic pathways including AMP-activated protein kinase (AMPK) and silent information regulator 1 (sirt1). Furthermore, pharmacological inhibition of the sirt1 or AMPK pathways attenuated these beneficial effects of Rb2 on hepatic autophagy and lipid accumulation. Taken together, these results suggested that Rb2 alleviated hepatic lipid accumulation by restoring autophagy via the induction of sirt1 and activation of AMPK, and resulted in improved nonalcoholic fatty liver disease (NAFLD) and glucose tolerance. |
| WOS关键词 | FATTY LIVER-DISEASE ; MOLECULAR-MECHANISMS ; INSULIN-RESISTANCE ; INDUCED APOPTOSIS ; PATHWAY ; MICE ; CELLS ; STRESS ; HEPATOSTEATOSIS ; IDENTIFICATION |
| 资助项目 | State Key Laboratory of Drug Research, National Natural Science Foundation[81473262] ; State Key Laboratory of Drug Research, National Natural Science Foundation[81503124] ; Science and Technology Commission of Shanghai Municipality Project[15431901000] ; Science and Technology Commission of Shanghai Municipality Project[14ZR1447700] ; Chinese Academy of Sciences[XDA12040311] ; Chinese Academy of Sciences[CASIMM0120162025] ; Chinese Academy of Sciences[CASIMM0120164014] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000404113900169 |
| 出版者 | MDPI AG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272678]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wang, He-Yao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Qi,Wang, Ting,Yang, Liu,et al. Ginsenoside Rb2 Alleviates Hepatic Lipid Accumulation by Restoring Autophagy via Induction of Sirt1 and Activation of AMPK[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(5). |
| APA | Huang, Qi,Wang, Ting,Yang, Liu,&Wang, He-Yao.(2017).Ginsenoside Rb2 Alleviates Hepatic Lipid Accumulation by Restoring Autophagy via Induction of Sirt1 and Activation of AMPK.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(5). |
| MLA | Huang, Qi,et al."Ginsenoside Rb2 Alleviates Hepatic Lipid Accumulation by Restoring Autophagy via Induction of Sirt1 and Activation of AMPK".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.5(2017). |
入库方式： OAI收割
来源：上海药物研究所
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