Simultaneous determination of eight Danshen polyphenols in rat plasma and its application to a comparative pharmacokinetic study of DanHong injection and Danshen injection
文献类型：期刊论文
| 作者 | Li, Xiuxue1,2; Du, Feifei1; Jia, Weiwei1; Olaleye, Olajide E.1; Xu, Fang1; Wang, Fengqing1; Li, Li1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2017-04 |
| 卷号 | 40期号:7页码:1470-1481 |
| 关键词 | DanHong injection Danshen injection pharmacokinetics polyphenols traditional Chinese medicine |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201601340 |
| 文献子类 | Article |
| 英文摘要 | Polyphenols derived from Danshen are responsible for the therapeutic effects of DanHong injection, a two-herb combination of Danshen and Honghua. Whether the pharmacokinetics of Danshen polyphenols is changed by coexisting Honghua constituents remains unknown. A sensitive ultra high performance liquid chromatography with tandem mass spectrometry methodwas developed in this study for simultaneous determination of eight Danshen polyphenols (i.e., protocatechuic aldehyde, protocatechuic acid, tanshinol, salvianolic acid D, rosmarinic acid, salvianolic acid A, lithospermic acid, and salvianolic acid B) in rat plasma and applied to a comparative pharmacokinetic study of DanHong injection and Danshen injection. Liquid chromatography conditions, mass spectrometry parameters, and sample preparation were optimized step by step. The calibration curves showed good linearity (r > 0.99) for all the polyphenols. The mean extraction efficiencies ranged from 62.2 to 88.7% with negligible matrix effects. The intrabatch and interbatch precision at all the quality control levels were less than 15% of the nominal concentrations with accuracy of 88.8-114%, except that precision and accuracy at lower limit of quantitation were 3.2-17.3 and 95.7-119%, respectively. Comparative pharmacokinetic study suggested that the coexisting Honghua constituents might have negligible influences on the pharmacokinetics of Danshen polyphenols from DanHong injection. The bioanalytical method could also be applied to pharmacokinetic studies of other Danshen herbal products. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SALVIANOLIC ACID-B ; 7 PHENOLIC-ACIDS ; MASS-SPECTROMETRY ; TISSUE DISTRIBUTION ; ARRAY DETECTION ; MILTIORRHIZA ; COMPONENTS ; HPLC ; CONSTITUENTS |
| 资助项目 | National Natural Science Foundation of China[81573828] ; National Science and Technology Major Project of China "Key New Drug Creation and Manufacturing Program"[2013ZX09201020001003] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000399782900005 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272718]  |
| 专题 | 上海药物代谢研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Li, Li |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 2.Tianjin Univ Tradit Chinese Med, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiuxue,Du, Feifei,Jia, Weiwei,et al. Simultaneous determination of eight Danshen polyphenols in rat plasma and its application to a comparative pharmacokinetic study of DanHong injection and Danshen injection[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(7):1470-1481. |
| APA | Li, Xiuxue.,Du, Feifei.,Jia, Weiwei.,Olaleye, Olajide E..,Xu, Fang.,...&Li, Li.(2017).Simultaneous determination of eight Danshen polyphenols in rat plasma and its application to a comparative pharmacokinetic study of DanHong injection and Danshen injection.JOURNAL OF SEPARATION SCIENCE,40(7),1470-1481. |
| MLA | Li, Xiuxue,et al."Simultaneous determination of eight Danshen polyphenols in rat plasma and its application to a comparative pharmacokinetic study of DanHong injection and Danshen injection".JOURNAL OF SEPARATION SCIENCE 40.7(2017):1470-1481. |
入库方式： OAI收割
来源：上海药物研究所
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