Dual anti-ischemic effects of rosmarinic acid n-butyl ester via alleviation of DAPK-p53-mediated neuronal damage and microglial inflammation
文献类型：期刊论文
| 作者 | Wu, Lei2,4; Wang, Hong-min1,3,4; Li, Jin-long1,3,4; Feng, Hong-xuan2,4; Zhao, Wei-min1,3 ; Zhang, Hai-yan2
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2017-04 |
| 卷号 | 38期号:4页码:459-468 |
| 关键词 | cerebral ischemia rosmarinic acid n-butyl ester SH-SY5Y neuroblastoma cells apoptosis death-associated protein kinase p53 microglia inflammation |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2016.156 |
| 文献子类 | Article |
| 英文摘要 | The discovery of efficacious anti-ischemic drugs remains a challenge. Recently we have found that rosmarinic acid n-butyl ester (RABE), a derivative of rosmarinic acid, significantly protects SH-SY5Y cells against oxygen glucose deprivation (OGD)-induced cell death. In the present study we simultaneously investigated the effects of RABE on the two key players in the pathophysiology of cerebral ischemia, ischemic neuronal damage and microglial inflammation. Pretreatment with RABE (1, 10 mu mol/L) dose-dependently attenuated OGD-or H2O2-induced reduction of the viability of SH-SY5Y neuroblastoma cells. RABE pretreatment concurrently reduced the apoptotic cell rate, down-regulated the expression of the pro-apoptotic proteins Bax and p53, and up-regulated the expression of the anti-apoptotic protein phosphorylated death-associated protein kinase (DAPK). Furthermore, pretreatment with RABE (3 mu mol/L) markedly inhibited lipopolysaccharide (LPS)-induced increases in the release of TNF-alpha, IL-1 beta, NO and PGE2, and the expression levels of iNOS, and COX-2 in cultured rat microglial cells. In conclusion, these results reveal for the first time the potential anti-ischemic effects of RABE on neuronal and glial cells and elucidate the molecular mechanisms involved in its dual beneficial profiles in vitro. RABE may be a promising drug lead/candidate for the treatment of ischemic stroke. |
| WOS关键词 | ACUTE ISCHEMIC-STROKE ; TISSUE-PLASMINOGEN ACTIVATOR ; BRAIN ISCHEMIA ; NITRIC-OXIDE ; DEATH ; PATHWAY ; NEUROTOXICITY ; ASSOCIATION ; APOPTOSIS ; DISEASE |
| 资助项目 | National Natural Science Foundation of China[81522045] ; National Natural Science Foundation of China[81473113] ; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China[SIMM1601KF-03] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5953656 |
| WOS记录号 | WOS:000399192700002 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272725]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第二研究室 |
| 通讯作者 | Zhao, Wei-min; Zhang, Hai-yan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Prod Chem, Shanghai 201203, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Lei,Wang, Hong-min,Li, Jin-long,et al. Dual anti-ischemic effects of rosmarinic acid n-butyl ester via alleviation of DAPK-p53-mediated neuronal damage and microglial inflammation[J]. ACTA PHARMACOLOGICA SINICA,2017,38(4):459-468. |
| APA | Wu, Lei,Wang, Hong-min,Li, Jin-long,Feng, Hong-xuan,Zhao, Wei-min,&Zhang, Hai-yan.(2017).Dual anti-ischemic effects of rosmarinic acid n-butyl ester via alleviation of DAPK-p53-mediated neuronal damage and microglial inflammation.ACTA PHARMACOLOGICA SINICA,38(4),459-468. |
| MLA | Wu, Lei,et al."Dual anti-ischemic effects of rosmarinic acid n-butyl ester via alleviation of DAPK-p53-mediated neuronal damage and microglial inflammation".ACTA PHARMACOLOGICA SINICA 38.4(2017):459-468. |
入库方式： OAI收割
来源：上海药物研究所
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