Evaluation of Endoproteinase Lys- C/Trypsin Sequential Digestion Used in Proteomics Sample Preparation
文献类型：期刊论文
| 作者 | Li Qian1,3; Feng Yu2; Tan Min-Jia1,3 ; Zhai Lin-Hui1
|
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-03 |
| 卷号 | 45期号:3页码:316-321 |
| 关键词 | Trypsin Lys-C Sequential digestion Proteomics Liquid chromatography-tandem mass spectrometry Missed cleavage |
| ISSN号 | 0253-3820 |
| DOI | 10.1016/S1872-2040(17)60998-8 |
| 文献子类 | Article |
| 英文摘要 | Endoproteinase lysine- C ( Lys- C)/ trypsin sequential digestion and trypsin digestion were used in 293T cell proteomics sample preparation and the differences of Lys- C/ trypsin sequential digestion and trypsin digestion in proteomics sample preparation were systematically evaluated. It was found that the number of identified peptides and proteins increased significantly and missed cleavage sites, especially K sites decreased dramatically through Lys- C/ trypsin sequential digestion. The average sequence coverage of identified proteins in Lys- C/ trypsin sequential digestion sample was higher than that in trypsin digestion sample. Besides, different amounts of enzymes were tested to select the optimal usage of enzymes in Lys- C/ trypsin sequential digestion. This study provided the references for proteomics sample preparation. |
| WOS关键词 | MASS-SPECTROMETRY ; PHOSPHOPROTEOMIC ANALYSIS ; BREAST-CANCER ; TRYPSIN ; YEAST ; QUANTIFICATION ; IDENTIFICATION ; PROTEINS |
| 资助项目 | Youth Science and Technology Talents in Shanghai Sail Plan of China[16YF1414000] ; National Natural Science Foundation of China[31370814] ; Shanghai Science and Technology Committee of China[14DZ2261100] ; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences[DOI: 10.1016/S1872-2040(17)60998-8] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5951421 |
| WOS记录号 | WOS:000398097200002 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272752]  |
| 专题 | 化学蛋白质组学研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 科研与新药推进处 |
| 通讯作者 | Zhai Lin-Hui |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Beijing Hualishi Sci Co Ltd, Beijing 101312, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li Qian,Feng Yu,Tan Min-Jia,et al. Evaluation of Endoproteinase Lys- C/Trypsin Sequential Digestion Used in Proteomics Sample Preparation[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(3):316-321. |
| APA | Li Qian,Feng Yu,Tan Min-Jia,&Zhai Lin-Hui.(2017).Evaluation of Endoproteinase Lys- C/Trypsin Sequential Digestion Used in Proteomics Sample Preparation.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(3),316-321. |
| MLA | Li Qian,et al."Evaluation of Endoproteinase Lys- C/Trypsin Sequential Digestion Used in Proteomics Sample Preparation".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.3(2017):316-321. |
入库方式： OAI收割
来源：上海药物研究所
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