Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases
文献类型：期刊论文
| 作者 | Li, Shuijun1,3; Jia, Jingying1; Liu, Gangyi1; Wang, Wei2; Cai, Yongbao2; Wang, Yiping3 ; Yu, Chen1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2008-07-01 |
| 卷号 | 870期号:1页码:63-67 |
| 关键词 | homocysteine LC-ESI-MS/MS cardiovascular disease method comparison |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2008.06.003 |
| 文献子类 | Article |
| 英文摘要 | A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the determination of human plasma homocysteine (Hcy), an important independent risk factor for cardiovascular disease, with a simplified sample pretreatment procedure and a zero blank free of endogenous Hcy for calibrator/QC preparation. Following protein precipitation, chromatographic separation was performed on Hypersil Aquasil C-18 column (50 mm x 2.1 mm, 5 mu m, Thermo) using mobile phase of aqueous 10% methanol containing 0.02% formic acid at 0.25 mL/min. Hcy and deuterated internal standard were detected in the multiple reaction monitoring mode with precursor to product ion transitions of m/z 136.1/90.0 and 140.1/94.0, respectively. The retention time was 1.2 min, and the total run time was 2 min per injection. A streamlined three-point calibration curve and one-point QC was used. Excellent linearity was observed with correlation coefficient (r) > 0.99. The intra- and inter-batch were <= 3.24% and <= 4.04%, and accuracy was within +/- 10%. Method comparison between the proposed method (y) and FPIA assay (x) demonstrated a correlation equation of y = 1.003x + 0.4318 (r = 0.9589). The developed method, improved for automation with cost-effective reagents, was proven to be suitable for high-throughput quantitative determination of Hcy in clinical practice by successfully applying it to the cardiovascular disease study. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; INDUCED FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; METHYLMALONIC ACID ; ASSAY ; PERFORMANCE ; CYSTEINE ; URINE ; SERUM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000257835000011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272880]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yu, Chen |
| 作者单位 | 1.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China; 2.Shanghai Xuhui Cent Hosp, Dept Cardiol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuijun,Jia, Jingying,Liu, Gangyi,et al. Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,870(1):63-67. |
| APA | Li, Shuijun.,Jia, Jingying.,Liu, Gangyi.,Wang, Wei.,Cai, Yongbao.,...&Yu, Chen.(2008).Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,870(1),63-67. |
| MLA | Li, Shuijun,et al."Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 870.1(2008):63-67. |
入库方式： OAI收割
来源：上海药物研究所
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