hTERT-targeted RNA interference inhibits tumorigenicity and motility of HCT116 cells
文献类型：期刊论文
| 作者 | Shen, Ying; Zhang, Yong-Wei; Zhang, Zhi-Xiang; Miao, Ze-Hong; Ding, Jian
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| 刊名 | CANCER BIOLOGY & THERAPY
 |
| 出版日期 | 2008-02 |
| 卷号 | 7期号:2页码:228-236 |
| 关键词 | hTERT siRNA telomerase adhesion motility integrin HGF/c-Met |
| ISSN号 | 1538-4047 |
| DOI | 10.4161/cbt.7.2.5259 |
| 文献子类 | Article |
| 英文摘要 | Telomerase is proposed as an anticancer target. Increasing evidence suggests that telomerase is involved in functions independent of telomere-extension activity. In this study, we designed a small interfering RNA (siRNA) targeting human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Using transient and persistent transfection of hTERT siRNA into telomerase-positive human colon carcinoma HCT116 cells, we demonstrated that hTERT siRNA suppresses hTERT expression and leads to inhibition of telomerase activity and HCT116 cell growth and in vivo tumorigenicity in nude mice. Further analysis indicated that depletion of hTERT reduces cell adhesion, migration, and invasion prior to inhibition of cell proliferation. Downregulation of hTERT also decreased the expression levels of adhesion- and motility-related proteins, in particular c-Met and integrins. The lack of detectable changes in telomere length showed that downregulation of hTERT in the present system had no significant effect on telomere-extension activity. Taken together, our results highlight the therapeutic potential of a novel hTERT siRNA and suggest that hTERT is involved in the regulation of cell motility in a telomere-independent manner. |
| WOS关键词 | TELOMERASE REVERSE-TRANSCRIPTASE ; HUMAN CANCER-CELLS ; MAMMALIAN-CELLS ; COLORECTAL-CARCINOMA ; GENE-EXPRESSION ; INVASIVE GROWTH ; DOWN-REGULATION ; TUMOR-GROWTH ; METASTASIS ; ANGIOGENESIS |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256658500012 |
| 出版者 | LANDES BIOSCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272987]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, Jian |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Div Anti Tumor Pharmacol, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Ying,Zhang, Yong-Wei,Zhang, Zhi-Xiang,et al. hTERT-targeted RNA interference inhibits tumorigenicity and motility of HCT116 cells[J]. CANCER BIOLOGY & THERAPY,2008,7(2):228-236. |
| APA | Shen, Ying,Zhang, Yong-Wei,Zhang, Zhi-Xiang,Miao, Ze-Hong,&Ding, Jian.(2008).hTERT-targeted RNA interference inhibits tumorigenicity and motility of HCT116 cells.CANCER BIOLOGY & THERAPY,7(2),228-236. |
| MLA | Shen, Ying,et al."hTERT-targeted RNA interference inhibits tumorigenicity and motility of HCT116 cells".CANCER BIOLOGY & THERAPY 7.2(2008):228-236. |
入库方式： OAI收割
来源：上海药物研究所
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