Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons
文献类型：期刊论文
| 作者 | Yu, Yong; Chen, Xue-qin; Cui, Yao-yuan; Hu, Guo-yuan |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2007-12 |
| 卷号 | 28期号:12页码:1891-1897 |
| 关键词 | action potential calcineurin cyclosporin A delayed rectifier K+ channel hippocampus tacrolimus |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2007.00730.x |
| 文献子类 | Article |
| 英文摘要 | Aim: The aim of the present study was to investigate the electrophysiological actions of cyclosporin A (CsA) and tacrolimus (FK506) on neurons in the brain, and to elucidate the relevant mechanisms. Methods: Whole-cell current-clamp recording was made in CA1 pyramidal neurons in rat hippocampal slices; whole-cell voltage-clamp recording was made in dissociated hippocampal CA1 pyramidal neurons of rats. Results: CsA (100 mu mol/L) and FK506 (50 mu mol/L) did not significantly alter the passive electrical properties of hippocampal CA1 pyramidal neurons, but slowed down the repolarizing phase of the action potential. CsA (10-100 mu mol/L) selectively inhibited the delayed rectifier K+ current (I-K) in a concentration-dependent manner. CsA did not affect the kinetic properties of I-K. Intracellular dialysis of CsA (100 mu mol/L) had no effect on I-K. The inhibition of I-K by CsA (100 mu mol/L) persisted under the low Ca2+ conditions that blocked the basal activity of calcineurin. Conclusion: CsA exerted calcineurin-independent inhibition on the I-K in rat hippocampal pyramidal neurons. Taken together with our previous finding with FK506, it is conceivable that the spike broadening caused by the immunosuppressant drugs is due to direct inhibition on the I-K. |
| WOS关键词 | CALCINEURIN-INDEPENDENT INHIBITION ; IMMUNOSUPPRESSANT FK506 ; ION CHANNELS ; K+ CURRENTS ; PHOSPHATASES ; MODULATION ; FK-506 |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3089013 |
| WOS记录号 | WOS:000251327800004 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273095]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Hu, Guo-yuan |
| 作者单位 | 1.Fudan Univ, Zhongshan Hosp, Dept Neurosurg, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Yong,Chen, Xue-qin,Cui, Yao-yuan,et al. Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons[J]. ACTA PHARMACOLOGICA SINICA,2007,28(12):1891-1897. |
| APA | Yu, Yong,Chen, Xue-qin,Cui, Yao-yuan,&Hu, Guo-yuan.(2007).Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons.ACTA PHARMACOLOGICA SINICA,28(12),1891-1897. |
| MLA | Yu, Yong,et al."Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons".ACTA PHARMACOLOGICA SINICA 28.12(2007):1891-1897. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。