Effects of scutellarin on PKC gamma in PC12 cell injury induced by oxygen and glucose deprivation
文献类型：期刊论文
| 作者 | Wei Xu; Ruo-Peng Zha; Wen-Yi Wang; Yi-Ping Wang
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2007-10 |
| 卷号 | 28期号:10页码:1573-1579 |
| 关键词 | scutellarin PC12 cells apoptosis lactic dehydrogenase intracellular calcium protein kinase C |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2007.00502.x |
| 文献子类 | Article |
| 英文摘要 | Aim: To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep). Methods: Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+](i) was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)gamma was determined using both RT-PCR and Western blotting. The translocation of PKC gamma was assayed by subcellular fractionation and Western blotting. Results: OGD-Rep injury significantly elevated the level of LDH release, [Ca2+](i), mRNA expression and the translocation of PKC gamma compared in the PC12 cells with those of the normal group. Scu (10-100 mu mol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+](i), the percent of apoptosis, and the translocation of PKC gamma. Conclusion: Scu inhibits the increase of [Ca2+](i) and the activation of PKC gamma, exerting protective effects against PC12 cell injury induced by OGD-Rep. |
| WOS关键词 | PROTEIN-KINASE-C ; FOCAL CEREBRAL-ISCHEMIA ; CORTICAL-NEURONS ; CALCIUM ; BRAIN ; APOPTOSIS ; MEMBRANES ; DEATH ; PATHOPHYSIOLOGY ; TRANSLOCATION |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3089042 |
| WOS记录号 | WOS:000249699600008 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273132]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yi-Ping Wang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei Xu,Ruo-Peng Zha,Wen-Yi Wang,et al. Effects of scutellarin on PKC gamma in PC12 cell injury induced by oxygen and glucose deprivation[J]. ACTA PHARMACOLOGICA SINICA,2007,28(10):1573-1579. |
| APA | Wei Xu,Ruo-Peng Zha,Wen-Yi Wang,&Yi-Ping Wang.(2007).Effects of scutellarin on PKC gamma in PC12 cell injury induced by oxygen and glucose deprivation.ACTA PHARMACOLOGICA SINICA,28(10),1573-1579. |
| MLA | Wei Xu,et al."Effects of scutellarin on PKC gamma in PC12 cell injury induced by oxygen and glucose deprivation".ACTA PHARMACOLOGICA SINICA 28.10(2007):1573-1579. |
入库方式： OAI收割
来源：上海药物研究所
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