Morphine inhibits doxorubicin-induced reactive oxygen species generation and nuclear factor kappa B transcriptional activation in neuroblastoma SH-SY5Y cells
文献类型：期刊论文
| 作者 | Lin, Xin; Li, Qing; Wang, Yu-Jun ; Ju, Ya-Wen; Chi, Zhi-Qiang; Wang, Min-Wei; Liu, Jing-Gen
|
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 406页码:215-221 |
| 关键词 | apoptosis doxorubicin (DOX) morphine nuclear factor kappa B reactive oxygen species SH-SY5Y cell |
| ISSN号 | 0264-6021 |
| DOI | 10.1042/BJ20070186 |
| 文献子类 | Article |
| 英文摘要 | Morphine is recommended as a first-line opioid analgesic in the pain management of cancer patients. Accumulating evidence shows that morphine has anti-apoptotic activity, but its impact on the therapeutic applications of antineoplastic drugs is not well known. The present study was undertaken to test the hypothesis that morphine might antagonize the pro-apoptotic activity of DOX (doxorubicin), a commonly used antitumour drug for the treatment of neuroblastoma, in cultured SH-SY5Y cells. In the present study we demonstrated that morphine suppressed DOX-induced inhibition of cell proliferation and programmed cell death in a concentration-dependent, and naloxone as well as pertussis toxin-irreversible, manner. Further studies showed that morphine inhibited ROS (reactive oxygen species) generation, and prevented DOX-mediated caspase-3 activation, cytochrome c release and changes of Bax and Bc1-2 protein expression. The antioxidant NAC (N-acetylcysteine) also showed the same effects as morphine on DOX-induced ROS generation, caspase-3 activation and cytochrome c release and changes in Bax (Bc1-2-associated X protein) and Bc1-2 protein expression. Additionally, morphine was found to suppress DOX-induced NF-kappa B (nuclear factor kappa B) transcriptional activation via a reduction Of I kappa B alpha (inhibitor of nuclear factor kappa B) degradation. These present findings support the hypothesis that morphine can inhibit DOX-induced neuroblastoma cell apoptosis by the inhibition of ROS generation and mitochondrial cytochrome c release, as well as by blockade of NF-kappa B transcriptional activation, and suggests that morphine might have an impact on the antitumour efficiency of DOX. |
| WOS关键词 | CYTOCHROME-C RELEASE ; INDUCED APOPTOSIS ; HYDROGEN-PEROXIDE ; TUMOR-GROWTH ; CASPASE-3 ACTIVATION ; OXIDATIVE STRESS ; IN-VITRO ; MECHANISM ; DEATH ; ALPHA |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000249181200004 |
| 出版者 | PORTLAND PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273152]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Liu, Jing-Gen |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Sheyang Pharmaceut Univ, Dept Pharmacol, New Delhi 110016, India |
| 推荐引用方式 GB/T 7714 | Lin, Xin,Li, Qing,Wang, Yu-Jun,et al. Morphine inhibits doxorubicin-induced reactive oxygen species generation and nuclear factor kappa B transcriptional activation in neuroblastoma SH-SY5Y cells[J]. BIOCHEMICAL JOURNAL,2007,406:215-221. |
| APA | Lin, Xin.,Li, Qing.,Wang, Yu-Jun.,Ju, Ya-Wen.,Chi, Zhi-Qiang.,...&Liu, Jing-Gen.(2007).Morphine inhibits doxorubicin-induced reactive oxygen species generation and nuclear factor kappa B transcriptional activation in neuroblastoma SH-SY5Y cells.BIOCHEMICAL JOURNAL,406,215-221. |
| MLA | Lin, Xin,et al."Morphine inhibits doxorubicin-induced reactive oxygen species generation and nuclear factor kappa B transcriptional activation in neuroblastoma SH-SY5Y cells".BIOCHEMICAL JOURNAL 406(2007):215-221. |
入库方式： OAI收割
来源：上海药物研究所
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