Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment
文献类型：期刊论文
| 作者 | Chen, Xiao-Lan; Lu, Gang; Gong, Ying-Xia; Zhao, Liang-Cai; Chen, Jie; Chi, Zhi-Qiang ; Yang, Yi-Ming; Chen, Zhong; Li, Qing-Lin; Liu, Jing-Gen
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| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2007-08 |
| 卷号 | 17期号:8页码:689-700 |
| 关键词 | hippocampus glucose morphine dependence learning and memory |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2007.63 |
| 文献子类 | Article |
| 英文摘要 | Dependence and impairment of learning and memory are two well-established features caused by abused drugs such as opioids. The hippocampus is an important region associated with both drug dependence and learning and memory. However, the molecular events in hippocampus following exposure to abused drugs such as opioids are not well understood. Here we examined the effect of chronic morphine treatment on hippocampal protein expression by proteomic analyses. We found that chronic exposure of mice to morphine for 10 days produced robust morphine withdrawal jumping and memory impairment, and also resulted in a significant downregulation of hippocampal protein levels of three metabolic enzymes, including Fe-S protein 1 of NADH dehydrogenase, dihydrolipoamide acetyltransferase or E2 component of the pyruvate dehydrogenase complex and lactate dehydrogenase 2. Further real-time quantitative PCR analyses confirmed that the levels of the corresponding mRNAs were also remarkably reduced. Consistent with these findings, lower ATP levels and an impaired ability to convert glucose into ATP were also observed in the hippocampus of chronically treated mice. Opioid antagonist naltrexone administrated concomitantly with morphine significantly suppressed morphine withdrawal jumping and reversed the downregulation of these proteins. Acute exposure to morphine also produced robust morphine withdrawal jumping and significant memory impairment, but failed to decrease the expression of these three proteins. Intrahippocampal injection of D-glucose before morphine administration significantly enhanced ATP levels and suppressed morphine withdrawal jumping and memory impairment in acute morphine-treated but not in chronic morphine-treated mice. Intraperitoneal injection of high dose of D-glucose shows a similar effect on morphine-induced withdrawal jumping as the central treatment. Taken together, our results suggest that reduced expression of the three metabolic enzymes in the hippocampus as a result of chronic morphine treatment contributes to the development of drug-induced symptoms such as morphine withdrawal jumping and memory impairment. |
| WOS关键词 | CENTRAL CHOLINERGIC SYSTEMS ; INDUCED PLACE PREFERENCE ; LONG-TERM POTENTIATION ; PROTEIN-KINASE-II ; SPONTANEOUS-ALTERNATION ; PHYSICAL-DEPENDENCE ; OPIATE WITHDRAWAL ; ACETYLCHOLINE-RELEASE ; RECEPTOR ANTAGONIST ; DORSAL HIPPOCAMPUS |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2844897 |
| WOS记录号 | WOS:000249774500005 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273184]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Liu, Jing-Gen |
| 作者单位 | 1.Zhejiang Univ, Sch Med, Dept Pharmacol, Hangzhou 310031, Peoples R China 2.Chinese Acad Sci, Inst Biol Sci, Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Anhui Univ Tradit Chinese Med, Dept Pharmacol, Hefei 230032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xiao-Lan,Lu, Gang,Gong, Ying-Xia,et al. Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment[J]. CELL RESEARCH,2007,17(8):689-700. |
| APA | Chen, Xiao-Lan.,Lu, Gang.,Gong, Ying-Xia.,Zhao, Liang-Cai.,Chen, Jie.,...&Liu, Jing-Gen.(2007).Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment.CELL RESEARCH,17(8),689-700. |
| MLA | Chen, Xiao-Lan,et al."Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment".CELL RESEARCH 17.8(2007):689-700. |
入库方式： OAI收割
来源：上海药物研究所
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