Requirement of PSD-95 for dopamine D-1 receptor modulating glutamate NR1a/NR2B receptor function
文献类型：期刊论文
| 作者 | Gu, Wei-hua; Yang, Shen; Shi, Wei-xing; Jin, Guo-zhang ; Zhen, Xue-chu
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| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2007-06 |
| 卷号 | 28期号:6页码:756-762 |
| 关键词 | D1 dopamine receptor NMDA PSD-95 signal transduction calcium |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2007.00557.x |
| 文献子类 | Article |
| 英文摘要 | Aim: To elucidate the role of scaffold protein postsynaptic density (PSD)-95 in the dopamine D-1 receptor (D1R)-modulated NR1a/NR2B receptor response. Methods: The human embryonic kidney 293 cells expressing D1R (tagged with the enhanced yellow fluorescent protein) and NR1a/NR2B with or without co-expression of PSD-95 were used in the experiments. The Ca2+ influx measured by imaging technique was employed to monitor N -methyl-D-aspartic acid receptors (NMDAR) function. Results: The application of dopamine (DA, 100 mu mol/L) did not alter glutamate/glycine (Glu/Gly)-induced NMDAR-mediated Ca2+ influx in cells only expressing the D1R/NR1a/NR2B receptor. However, DA increased Glu/Gly-induced Ca2+ influx in a concentration-dependent manner while the cells were co-expressed with PSD-95. D1R-stimulated Ca2+ influx was inhibited by a selective D1R antagonist SCH23390. Moreover, pre-incubation with either the protein kinase A (PKA) inhibitor H89, or the protein kinase C (PKC) inhibitor chelerythrine attenuated D1R-enhanced Ca2+ influx induced by the N-methyl-D-aspartic acid (NMDA) agonist. The results clearly indicate that D1R-modulated NR1a/NR2B receptor function depends on PSD-95 and is subjected to the regulation of PKA and PKC. Conclusion: The present study provides the first evidence that PSD-95 is essential in D1R-regulated NR1a/NR2B receptor function. |
| WOS关键词 | D-ASPARTATE RECEPTOR ; NMDA RECEPTOR ; D1 RECEPTOR ; DESENSITIZATION ; SUBUNITS ; PATHWAY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2845666 |
| WOS记录号 | WOS:000246756300002 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273233]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Shi, Wei-xing |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Dept Pharmacol, Shanghai 201203, Peoples R China 2.Yale Univ, Sch Med, Dept Psychiat, Neuropsychopharmacol Res Unit, New Haven, CT 06511 USA |
| 推荐引用方式 GB/T 7714 | Gu, Wei-hua,Yang, Shen,Shi, Wei-xing,et al. Requirement of PSD-95 for dopamine D-1 receptor modulating glutamate NR1a/NR2B receptor function[J]. ACTA PHARMACOLOGICA SINICA,2007,28(6):756-762. |
| APA | Gu, Wei-hua,Yang, Shen,Shi, Wei-xing,Jin, Guo-zhang,&Zhen, Xue-chu.(2007).Requirement of PSD-95 for dopamine D-1 receptor modulating glutamate NR1a/NR2B receptor function.ACTA PHARMACOLOGICA SINICA,28(6),756-762. |
| MLA | Gu, Wei-hua,et al."Requirement of PSD-95 for dopamine D-1 receptor modulating glutamate NR1a/NR2B receptor function".ACTA PHARMACOLOGICA SINICA 28.6(2007):756-762. |
入库方式： OAI收割
来源：上海药物研究所
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