Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry
文献类型：期刊论文
| 作者 | Li, Xiaochuan; Yu, Chen; Wang, Li; Lu, Youli; Wang, Wenyi; Xuan, Lijiang ; Wang, Yiping
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2007-04-11 |
| 卷号 | 43期号:5页码:1864-1868 |
| 关键词 | lithospermic acid B metabolites LC/MS/MS pharmacokinetic |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2007.01.004 |
| 文献子类 | Article |
| 英文摘要 | A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the simultaneous determination of lithospermic acid B and its three main O-methylated metabolites in rat serum with silibinin as the internal standard. The calibration curves for LSB, and the three metabolites were linear over the ranges of 16-4096, and 8-2048 ng/ml, respectively, with coefficients of correlation > 0.998. For LSB, the intra-assay coefficient of variance (CV) was less than 9.3% and the inter-assay CV was less than 8.9%. The inter-assay mean accuracy was between 92.8% and 104.7%. For the three metabolites, the intra-assay CV was less than 8.7% and the inter-assay CV was less than 9.9%. The inter-assay mean accuracy was between 92.5% and 107.9%. This quantitation method was successfully applied to a pharmacokinetic study of LSB in rats. Also, a total recovery of 5.2% was found in bile after oral administration. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS关键词 | SALVIA-MILTIORRHIZA ; ACTIVE COMPONENTS ; RAT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000246008300040 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273287]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wang, Yiping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaochuan,Yu, Chen,Wang, Li,et al. Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(5):1864-1868. |
| APA | Li, Xiaochuan.,Yu, Chen.,Wang, Li.,Lu, Youli.,Wang, Wenyi.,...&Wang, Yiping.(2007).Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,43(5),1864-1868. |
| MLA | Li, Xiaochuan,et al."Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 43.5(2007):1864-1868. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。