Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase
文献类型：期刊论文
| 作者 | Liu, Weizhi; Du, Li; Zhang, Liang; Chen, Jing ; Shen, Xu; Jiang, Hualiang
|
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2007-03 |
| 卷号 | 52期号:1页码:74-81 |
| 关键词 | Helicobacter pylori fatty acid biosynthesis acyl carrier protein (ACP) beta-hydroxyacyl-ACP dehydratase (HpFabZ) GST-pull down surface plasmon resonance (SPR) antibacterial agents |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2006.09.003 |
| 文献子类 | Article |
| 英文摘要 | Acyl carrier protein (ACP) is an essential component in the type II fatty acid biosynthesis (FAS II) process and is responsible for the acyl group transfer within a series of related enzymes. In this work, the ACP from Helicobacter pylori strain SS I was cloned and the gene sequence of Hpacp was deposited in the GenBank database (Accession No.: AY904356). Two forms of HpACP (apo, holo) were successfully purified and characterized. The thermal stability of these two forms was quantitatively investigated by CD spectral analyses. The results revealed that the holo-HpACP was more stable than apo-HpACP according to the transition midpoint temperature(T-m). Moreover, the interaction of HpACP with the related enzyme (beta-hydroxyacyl-ACP dehydratase, HpFabZ) was determined by GST-pull down assay and surface plasmon resonance (SPR) technique in vitro, the results showed that HpACP displays a strong binding affinity to HpFabZ (K-D = 1.2 x 10(-8) M). This current work is hoped to supply useful information for better understanding the ACP features of Helicobacter pylori SS I strain. (c) 2006 Elsevier Inc. All rights reserved. |
| WOS关键词 | FATTY-ACID BIOSYNTHESIS ; ESCHERICHIA-COLI ; MYCOBACTERIUM-TUBERCULOSIS ; STREPTOMYCES-COELICOLOR ; PLASMODIUM-FALCIPARUM ; CIRCULAR-DICHROISM ; SARS CORONAVIRUS ; SYNTHASE-III ; TRANSACYLASE ; INHIBITOR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244772900010 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273317]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物发现与设计中心 |
| 通讯作者 | Shen, Xu |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Weizhi,Du, Li,Zhang, Liang,et al. Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase[J]. PROTEIN EXPRESSION AND PURIFICATION,2007,52(1):74-81. |
| APA | Liu, Weizhi,Du, Li,Zhang, Liang,Chen, Jing,Shen, Xu,&Jiang, Hualiang.(2007).Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase.PROTEIN EXPRESSION AND PURIFICATION,52(1),74-81. |
| MLA | Liu, Weizhi,et al."Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase".PROTEIN EXPRESSION AND PURIFICATION 52.1(2007):74-81. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。